Studying of Histamine Concentration and Quality Parameters in Some Local Soft cheese and Imported Processed Cheese Types in Basrah Markets 

Awatif Hameed Issa1, Shamaail Abdulaali Saewan2 & Bassima Abdulrazzaq Abdulrahman3

 1Dept. of Biology, College of Science,

2Dept. of Food Science, College of Agriculture,

3Dept. of Ecology, College of Science, University of Basra, Basra-Iraq. 


Histamine  is an important biogenic amine, formed by decarboxylation of free amino acid histidine. Presence of histamine in food causes allergy to some peoples, so it is important to determine level of this compound in foods like cheese. This study was carried out to extract and estimate histamine in nine local and imported cheese categories in Basrah city markets, by using ELISA. The mean concentrations of histamine in cheese samples ranged from (0.2725 - 23.5025) mg/Kg in Arab cheese and Dhafayer cheese respectively. the results showed that concentrations of histamine didn’t exceed permitted level of 100-200 mg/kg. The study also conducted to ensure that the cheese products conform to the Iraqi standard for canned foods.

Key Words: Histamine,  Cheese,  Biogenic amines,  ELISA


[1] Compagnone, D.; Isoldi, G.; Moscone, D. and Palleschi, G. "Amperometric detection of biogenic amines in cheese using immobilised diamine oxidase", Analytical letters, Vol. (34), No. 6, pp. 841–854. (2001).

[2] Vallejos, M. J. M.; Pham, L. J. and Barraquio, V. L. "Biogenic amines in some natural and processed cheeses sold in Laguna province, Philippines", Philippine Journal of Science. Vol. (141), No. 1, pp. 111-115. (2012).

[3] Al-Abdulnebi, S. A. S. “Estimation of some biogenic amines, hydrocarbons and trace elements in the muscles of  fresh, frozen and canned fishes, A thesis. College of Agriculture, University of Basrah, Iraq, 185 p. (2013).

[4] Stratton, J. E.; Hutkins, R. W.; Taylor, S. L. "Biogenic amines in cheese and other fermented foods", A review. Journal of Food Protection. Vol. (54), No. 6, pp. 460 – 470. (1991).

[5] Huss, H. H.; Ababouch, L. and Gram. “Assessment and management of seafood safety and quality, Chapter 5. FAO Rome. (2004).

[6] Casella, I. G.; Gatta, M. and Desimoni, E. "Determination of histamine by high-pH anion-exchange chromatography with electrochemical detection",Food Chemistry. Vol. (73), No. 3, pp. 367-372. (2001).

[7] Santos M. H. S. “Biogenic amines: their importance in foods, A review.  International Journal of  Food Microbiology. Vol. (29), pp. 213-231. (1996).

[8] Ekici, K; Coskun, H. and  Sienkiewicz, T. “Histamine formation and its control in cheese, A review. Journal of Food Technology. Vol. (3), No. 1, pp 60-63. (2005).

[9] Russo, P.; Spano, G.; Arena, M. P.; Capozzi, V.; Grieco, F. and Beneduece, L. Are consumers aware of the risks related to biogenic amines in food?, Current Research Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, pp. 1087–1095. (2010).

[10] Doeglas, H. M. G., Huisman, J. and Nater, J. P. Histamine intoxication after cheese, Lancet, 2, 1361. In: Toxins in food, p. 135-136. (1964).

[11]  Ehsani, A.;  Mahmoudi, R.; Khodayari, M.;  and Zare, P. "Histamine levels in 3 types of Iranian cheese by Ion-exchange chromatography", Walailak J. Sci. and Tech. , Vol. (9),  No 3, pp 281285. (2012).

[12] Švarc-Gajic´, J. and Stojanovic´, Z. "Determination of histamine in cheese by chronopotentiometry on a thin film mercury electrode", Food Chemistry, Vol. (124), pp. 1172–1176. (2011).

[13] Rohani1, S. M. R.; Aliakbarlu, J.; Ali Ehsani, A. and Hassanzadazar1, H. "Biogenic amines determination in some traditional cheeses in West Azerbaijan province of Iran", Veterinary Research Forum. Vol. (4), No. 2, pp. 115 – 118. (2013).

[14] Al-Khaeun, S. N. A. Estimation of histamine in some local food and detection of histidine genes (hdc) from their isolated bacteria and it’s role in accumulation of histamine”, A thesis. College of Agriculture, University of Basrah, 210 p. (2016).  

[15] Canadian Food Inspection Agency (CFIA) “Guide to food Labelling and Advertising, Canada. 447p. (2010).

[16] Gomes, S.; Nogueira, M. and Ferreira, M. "Portuguese consumers’ attitudes towards food labeling", Chapter 1. WHO. Regional Office for Europe. Denmark. 50 p. (2017).

[17] ICOSQC; Iraqi Central Organization for Standardization and quality control, IQS 230. Food labeling for packaging and canning food, Iraq, (In Arabic). (1989).

[18] ICOSQC; "Iraqi Central Organization for Standardization and quality control, IQS 1847. Expiry dates of foods". Iraq .(In Arabic). (1999).

[19] Steel, R. G. D.; Torrie, J. H. and Dickey, D. A. “Principles and procedures of statistics, A biometrical approach. 3rd ed. McGraw Hill Book Company Inc, New York, USA. (1996).

[20] Pinho, O.;  Ferreira, I. M. P. L. V. O;  Mendes, E.;  Oliveira, B. M. and  Ferreira, M. "Effect of temperature on evolution of free amino acid and biogenic amine contents during storage of Azeitão cheese". Food Chemistry, Vol. (75), No. 3, pp. 287–291. (2001).

[21] Standarová, E.; Borkovcová, I.; Dušková, M. ; Přidalová, H.; Dračková, M.; and Vorlová, L. "Effect of some factors on the biogenic amines and polyamines content in blue-veined cheese niva", Czech J. Food Sci., Vol. (27), pp. 410-413. (2009).