Flow- Injection Spectrophotometric Determination of some Sulpha Drugs via Oxidative Coupling with 4-Amino-N, N-diethylaniline. Application to Various Samples


 Yousif J. Azeez

College of Science, Salahaddin University


Abstract
A flow injection spectrophotometric method is described for the determinaiton of sulphonamides
in aqueous and biological samples. The method is based on the oxidative coupling reaction of 4-amino-N,
N-diethylaniline with sulphonamide drugs in the presence of potassium dichromate as oxidizing agent.
Intense violet, water soluble dye was obtained which has a maximum absorption at 550nm,. Linear
calibration graphs were obtained for different sulphonamides with a molar absorpitivity of 4200-5270 l mol-1 cm-1
and a relative standard deviation less than 0.56%. The proposed method has proved to be highly sensitive (detection limit range 0.30-0.63 μg ml-1  sulphonamide) and accurate (recovery % was 98.2-101.2%). The nature of the coloured dye and its stability constant were determined. The method was applied successfully to the determination of sulphonamide drugs in pharmaceutical preparations, blood and urine samples.Keywords: FIA, Spectrophotometer 4-amino-N, N-diethylaniline

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