Characterization of Durum Wheat Genotypes by Seed Storage- Protein Electrophoresis


Sherwan I. Tofiq, Aram A.Muhammed, Taban N. Hamma Ameen

College of Agriculture, University of Sulaimani



Abstract:
Durum wheat grains from twenty five genotypes of 5x5 diallel crosses, which includes
20 crosses and 5 parents, were collected. Analyzing of seed storage –proteins for all
genotypes done by sodium dodcyle sulphate polyacrylamide gel electrophoresis(SDS-
PAGE). Evaluation of Genetic composition for HMW and LMW of glutenin and gliadin
unit bands were done by comparing the results and number of bands and pair bands with
the reference standards, results showed high rate of quality in addition to adaptation to
local environment, results supported by measuring protein % and Ash% and color
pigments.

Keywords: Durum wheat genotypes, SDS-PAGE, HMW, LMW, Color pigments.

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