Sequence Verifications and Promoter Analysis of The Prolactin Gene


Beston F. Nore1, Tayfoor Jalil Mahmoud2, Ban Mousa Rashid3

School of Pharmacy, University of Sulaimani, 2 College of Medicine, Hawler Medical University, 3 School of Pharmacy, University of Sulaimani



Abstract:
Prolactin or luteotropic hormone (PRL) is a protein hormone. Hyperprolactinemia is
one of the most common endocrine disorders. We initiate to investigate if the genetic
factor was behind some of the infertile cases suffering from hyperprolactinemia in slemani
city. For this study, 36 samples from infertile women were taken and the genomic DNA
was isolated. We desgined specific polynucleotide primers to amplify a portion of the PRL
gene using PCR technique. The PCR products were subjected for DNA sequencing
together with PCR products of 15 samples from healthy individuals. Silent mutations were
found between individuals, but non of them correlated to the PRL gene variations between
healthy and hyperprolactinemic cases. We perform promoter analysis of the PRL gene
using Bioinformatics approcach to identify/determine specific transcription regulator(s) of
the PRL gene.
Keywords: Prolactin, hyperprolactinemia, infertility, gene expression, promoter analysis.

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