Identification of Animal Species in Meat Broth by Simplex and Multiplex PCR

Peshnyar Muhammad Atta Rashid, Muhamad Omer Babashekh, Ari Salahadin Marouf, Kamaran Mohamad Amin

Molecular diagnostic laboratory, Sulaimani Veterinary directorate, Sulaimani  




Abstract:
In some country, some restaurants may use fraudulent substitution in meat product that has
less commercial value or undesirable. Effective laboratory tests must be available for prevention
of this phenomenon. In this study meat broth sample was successfully used to identify seven
animal species in cooked meals by simplex and multiplex PCR using species specific primers for
conserved region of mitochondrial DNA (mtDNA). The results showed effective amplification of
mtDNA without cross reaction between primers. Whereas existing methods require mincing and
homogenization of direct meat samples, this meat broth sampling method can be performed
directly and is thus easier to perform. In conclusion, meat broth sample could be used instead of
direct meat tissues for species identification of cooked meat in restaurants to control
adulteration substitutions of undesirable meat and protection of the customer rights

Keywords: Meat broth sample; species specific primer; veterinary forensic medicine; meat
identification



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