Detection of Extended Spectrum Beta-lactamase gene production by E. coli isolated from human and broiler in Sulemania province/ Iraq


Nahla M. Saeed
Department of Microbiology, College of Veterinary Medicine, University of Sulemania.





Abstract
A total of 311 Escherichia coli isolated from 460 healthy chickens at marketing age, and
56 E. coli isolated were obtained from 124 adult workers in broiler farms between April and
October 2013 -. Isolated E. coli were identified by cultural, biochemical, and Api E 20 test.
Antimicrobial susceptibility testing was determined by using the modified Kirby-Bauer’s disc
diffusion method. The antibiotic sensitivity result showed that the isolates were highly resistant
to Beta-lactam antibiotics. Among the 311 isolated E. coli from healthy chickens 264 (84.9%)
produced Beta-lactamase, while only 35 (62. 5%) of the 56 human isolates were found to
produce the enzyme. All of the 264 β-lactamase producer E. coli isolated from healthy chickens
and 35 β-lactamase producer E. coli isolated from human were tested for extended spectrum -
β-lactamase (ESBL) production, it was found that only 201 (76.1%) and 19 (54.3%), were
ESBL producers of E. coli isolated from healthy chickens and human respectively. Plasmids
were detected in all ESBL producer- E. coli (201) isolated from healthy chickens, which were
highly resistant to Beta-lactam antibiotics, while plasmid was detected only in 15 (78.9%) from
19 ESBLs producer E. coli isolated from human. By transformation the extracted plasmids into
E. coli DH5α; it was found that 165 (82.1%) Beta-lactamase genes from 201 isolates from
healthy chickens were carried by plasmid, while 10 (66.6%) Beta-lactamase genes from 15
plasmid isolated from human were carried by plasmid. The majority of these ESBL genes
(69.7%) were blaTEM and (10.9%) were blaCTX-M genes in healthy chickens, while in human the
majority of ESBL genes were (60%) blaTEM and (20. %) were blaCTX-M genes. Eleven isolated
from healthy chickens and one isolated from human did not contain blaTEM, blaSHV or blaCTX-M.

Keywords: - E. coli ; healthy chicken; human; Beta-lactamase; ESBL gene, plasmid
isolation.



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