Genetic characterization of peste des petitis ruminants virus (PPRV) from Sulaimani/ Iraq by phylogenetic analysis and sequencing of nucleoprotein and fusion protein gene


1 Muhamad omer Babashekh, 2 Peshnyar Muhammad Atta Rashid; 3 Ari Salahadin Marouf, 4 Zhino Hussen Raheem, 5 Kamaran Mohamad Amin
Molecular diagnostic laboratory, Sulaimani Veterinary directorate, Sulaimani




Abstract
An outbreak of peste des petitis ruminants (PPR) in sheep was studied in Sulaimani/
Iraqi Kurdistan Region. The study provided the first molecular characterization of the PPRV
lineage associated with fatal PPR infections in small ruminats. RT-PCR was used for diagnosis
of PPRV outbreaks during 2012-2013 and the phylogenetic analysis results based on F and N
genes. It was observed that three tested samples were positive for PPR, from which the two F
genes (ppr/Kurdistan/2012, ppr2/Kurdistan/2012) were genetically close to the (KF478924 &
JF274480) strain (turkey and Egypt) of lineage IV, respectively with 99% nucleotide sequence
homogeneity. Regarding N gene, it was noticed that one sample (ppr3/Kurdistan/2013) where
genetically close to (FJ795511, DQ840197 & DQ840190) strain (Emirate, Saudi Arabia & Israel)
%99, %98 and %97 respectively. The current study suggested that there are at least two
sources of PPRV in Iraq.

Keywords: PPRV; Sulaimani; peste des petits ruminants; Kurdistan



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