Cytological and Histological Effect of Paracetamol on the Testis and Liver in Albino Mice Mus musculus


Qader S. W. and Othman G. O.
Department of Biology-College of Education, University of Salahaddin




Abstract
The purpose of this study is to evaluate the acute toxicity effects of pure paracetamol tablets
on laboratory mice. In this study Mus musculus mice (6-8) weeks old were used. The toxicity effects
were evaluated on fifteen mice, in which they were divided equally into three groups, each one
consists of five individual mice. The first group was labeled as a vehicle group and received 5mL/kg
DW. The second and third groups were labeled as experimental groups which received 2 g/kg and 5
g/kg of paracetamol respectively. After fifteen days the animals were examined for toxicity signs and
then the liver and testis were processed for histological examinations. Furthermore, the sperm
abnormalities were recorded. The results of this study demonstrated that the low dose did not show
any morphological changes while some changes such as shivering, nausea and mortality were
manifested after administration of high dose of paracetamol. Many histological changes have been
observed after high dose treatment of paracetamol, the frequencies of abnormal sperm were
increased as well. Therefore, Paracetamol, which is believed to be a strong pain killer for the
hangover headache may damage liver and testis when consumes beyond the recommended dose.

Keywords: Paracetamol; Histology; Hepatotoxicity; Testicular toxicity and Sperm abnormalities



References
1. Vidhya Malar, H., Beware of Paracetamol Toxicity. Journal of Clinical Toxicology, 2012.
2(12): p. 2161-0495.
2. Graham, G.G., K.F. Scott, and R.O. Day, Alcohol and paracetamol. Australian Prescriber,
2004. 27(1): p. 14-15.
3. Bessems, J.G. and N.P. Vermeulen, Paracetamol (acetaminophen)-induced toxicity:
molecular and biochemical mechanisms, analogues and protective approaches. CRC
Critical Reviews in Toxicology, 2001. 31(1): p. 55-138.
4. Subich, D., Pharmaceutical drug sample tracking and control method. 2001, Google Patents.
5. Beasley, R., et al., Association between paracetamol use in infancy and childhood, and
risk of asthma, rhinoconjunctivitis, and eczema in children aged 6–7 years: analysis from
Phase Three of the ISAAC programme. The Lancet, 2008. 372(9643): p. 1039-1048.
6. Télez, M., et al., In vitro and in vivo evaluation of the antihypertensive drug atenolol in
cultured human lymphocytes: effects of long-term therapy. Mutagenesis, 2000. 15(3): p.195-202.
7. AL-Khan, H.I., Effect of Aqueous Extract of Capparis spinosa on Serum Antioxidant
Status in Paracetamol Treated Rats. Jou. Raf. Sci.,2010. 21(2): 29-36
8. McGill, M.R., et al., The mechanism underlying acetaminophen-induced hepatotoxicity in
humans and mice involves mitochondrial damage and nuclear DNA fragmentation. The
Journal of clinical investigation, 2012. 122(4): p. 1574-1583.
9. Marzouk, M., A.A. Sayed, and A.M. Soliman, Hepatoprotective and antioxidant effects of
Cichorium endivia L. leaves extract against acetaminophen toxicity on rats. Journal of
Medicine and Medical Sciences, 2011. 2(12): p. 1273-1279.
10. Abreu, R.V. and T. Moraes‐Santos, The Protective Effect of Coffe Against Paracetamol-
Induced Hepatic Injury In Rats. Journal of Food Biochemistry, 2011. 35(6): p. 1653-1659.
11. Ekaluo, U.B., E.V. Ikpeme, and A.E. Udokpoh, Sperm head abnormality and mutagenic
effects of aspirin, paracetamol and caffeine containing analgesics in rats. The Internet
Journal of Toxicology, 2009. 7(1): p. 1-9.
12. Tanaka, E., K. Yamazaki, and S. Misawa, Update: the clinical importance of
acetaminophen hepatotoxicity in non‐alcoholic and alcoholic subjects. Journal of clinical
pharmacy and therapeutics, 2000. 25(5): p. 325-332.
13. Tietz, N., A. Rinker, and L. Shaw, IFCC methods for the measurement of catalytic
concentration of enzymes Part 5. IFCC method for alkaline phosphatase
(orthophosphoric-monoester phosphohydrolase, alkaline optimum, EC 3.1. 3.1). Journal
of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und
klinische Biochemie, 1983. 21(11): p. 731-748.
14. Saleem, M.A. and M.S. Al-Attar, Protective Effects of Mentha Spicata Aqueous Extract
Against Ifosfamide induced Chromosomal Aberrations and Sperm abnormalities in male
Albino Mice. Trends in Biotechnology Research, 2013. 2: (1): 17-23.
15. Rumack, B.H. and H. Matthew, Acetaminophen poisoning and toxicity. Pediatrics, 1975.
55(6): p. 871-876.
16. Zezulka, A. and N. Wright, Severe metabolic acidosis early in paracetamol poisoning.
British medical journal (Clinical research ed.), 1982. 285(6345): p. 851.
17. James, L.P., P.R. Mayeux, and J.A. Hinson, Acetaminophen-induced hepatotoxicity. Drug
Metabolism and Disposition, 2003. 31(12): p. 1499-1506.
18. James, L.P., et al., Acetaminophen toxicity in mice lacking NADPH oxidase activity: role
of peroxynitrite formation and mitochondrial oxidant stress. Free radical research, 2003.
37(12): p. 1289-1297.
19. Yano, C. and H. Dolder, Rat testicular structure and ultrastructure after paracetamol
treatment. Contraception, 2002. 66(6): p. 463-467.
20. Pereira, F. and F.G. Brasileiro, Patologia ambiental. Brasileiro Filho G. et al. Bogliolo
Patologia. 6ed. Rio de Janeiro: Guanabara Koogan, 2000: p. 233-53.
21. Monsees, T., et al., Sertoli cells as a target for reproductive hazards. Andrologia, 2000.
32(4‐5): p. 239-246.