Cytological and Histological Effect of Paracetamol on the Testis and Liver in Albino Mice Mus musculus

Qader S. W. and Othman G. O.
Department of Biology-College of Education, University of Salahaddin

The purpose of this study is to evaluate the acute toxicity effects of pure paracetamol tablets
on laboratory mice. In this study Mus musculus mice (6-8) weeks old were used. The toxicity effects
were evaluated on fifteen mice, in which they were divided equally into three groups, each one
consists of five individual mice. The first group was labeled as a vehicle group and received 5mL/kg
DW. The second and third groups were labeled as experimental groups which received 2 g/kg and 5
g/kg of paracetamol respectively. After fifteen days the animals were examined for toxicity signs and
then the liver and testis were processed for histological examinations. Furthermore, the sperm
abnormalities were recorded. The results of this study demonstrated that the low dose did not show
any morphological changes while some changes such as shivering, nausea and mortality were
manifested after administration of high dose of paracetamol. Many histological changes have been
observed after high dose treatment of paracetamol, the frequencies of abnormal sperm were
increased as well. Therefore, Paracetamol, which is believed to be a strong pain killer for the
hangover headache may damage liver and testis when consumes beyond the recommended dose.

Keywords: Paracetamol; Histology; Hepatotoxicity; Testicular toxicity and Sperm abnormalities

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