Isolation, Identification and Pathogenicity of Seed borne fungi of some barley cultivars


1 Nadeem A. Ramadan, 2 Taha J. O. Zrary
1 Biology Dept. Coll. of Sci. Mosul University, 2 Biology Dept. Coll. of Sci. University of Koya






Abstract:
One hundred seeds were tested for each cultivars according to ISTA technique and
using agar plate method. The pathogenicity test was conducted to study the effect of 6
fungal pathogen on pre and post emergence damping off and survival damping off seedling
by inoculation of soil .Seven fungal genera Alternaria,Aureobasidium, Cladosporium,
Drechslera, Penicillium , Rhizoctonia and Stemphylium were isolated from Barley seeds
from Hawler governorate at season 2006. The Alternaria isolated at highest occurrence
percentage (%75.2),while the Stemphylium isolated record the lowest incidence percentage
(%0.1).Six fungal genera Alternaria, Aureobasidium, Cladosporium , Drechslera,
Rhizoctonia and Stemphylium were isolated from Barley seeds at the season of 2007. The
results obtained that the control significantly increases pre- emergence damping off
seedlings after 10 days of sowing of seeds, the mean value was (1.08) as compared with
fungal pathogen treatment which decreases their plant mean values obtained for
Alternaria,Aureobasidium, Curvularia, Drecslera, Stemphylium and Rhizoctonia were
recoded respectively .The ICARDA cultivar significantly affected which increased the post
damping off seedlings their value was (3.0476) as compare with other cultivars. The results
indicate that survival significantly increased their values were 7.833 as compared with
control 8.916.

Keywords: Barley, Seed health testing, Fungi



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