DNA profiling of F1 Hybrid in Bambara Groundnut (Vigna subterranea L. Verdc.) using Simple Sequence Repeat Marker


Nariman S. Ahmad
Field Crops Department, Faculty of Agricultural Sciences, Sulaimani University




Abstract
Bambara groundnut (Vigna subterranea L. Verdc.) is an indigenous crop of
African origin. It is considered as a drought tolerant and the third most important
leguminous crop in Sub-Saharan Africa due to its agronomic and nutritional potential.
Screening of presumed hybrids and progenies with molecular marker proved to be more
accurate and reliable compared to morphological characterizations. Testing the
accuracy of presumed hybrids resulted from the cross of African and Indonesia
landraces were performed using 12 SSR markers. Out of five crosses only one hybrid
resulted from the cross BC x S19 was confirmed to be genuine with four polymorphic
markers. The seeds of other crosses were found to come from the self-pollination of
maternal parent. These results clearly demonstrate that SSR markers should be useful
for rapid identification of true hybrid at the early stage of growth. Hence, the technique
contributes to genotype the progenies more accurately to develop genetic mapping and
QTL analysis, aiming towards more efficient utilization, conservation and development
of bambara groundnut germplasm.

Keywords: Bambara groundnut, SSR marker, capillary electrophoresis, F1 hybrid



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