Molecular Characterization for Karadi Sheep Breed Using Random Amplified Polymorphic DNA Markers

1 Yousif M. S. N. Al-Barzinji, 2 Muqdad K. Ali
1 Dept. of Animal Resource, College of Agriculture, University of Salahaddin, 2 Dept. of Animal Production, Faculty of Agricultural Science, University of Sulamania

RAPD-DNA markers were used to study genetic characterization among four
locations for Karadi sheep breed in Sulamania governorate. A total of 40 samples were
typed using twenty RAPD primers. Nine out of the twenty primers had clear bands,
which used to investigate the genetic variations among four locations(Halabjai Taza,
Penjwen, Halabja and Sharbazer) of same breed. Out of the nine primers 8 of them are
polymorphisms. A total of 119 bands were scored, of which 27 bands (25.05%) were
polymorphic and seven of polymorphic band were unique bands. For all location, Nei’s
gene diversity, Shannon index and percentage of polymorphic loci are respectively
averaged of 0.2361, 0.3415 and 34.60. Using unweighted pair-group method with
arithmetic average (UPGMA) dendrogram, the three clusters, the 1st cluster branch
consisted of the Karadi sheep in location 1 and 3, the 2nd cluster was including Karadi
sheep from location 2 and the 3rd one including sheep from location 4. These results
indicated that the Karadi sheep in location 1 and 3 is most genetically distant from the
Karadi sheep in location 2 .The dendrograms show that there are moderately genetic
diversity among Karadi sheep breed, were ranged from 0.251to 0.541. Based on the high
degree of genetic distance among the four locations it is concluded that there are widely
area for selection in this breed of sheep.

Keyword: Karadi sheep breed, Genetic distance, RAPD-PCR.


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