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Isolation, Molecular Identification and in vitro Antibiotic Susceptibility Testing of Mycoplasma agalactiae From Goats in Two Provinces of Kurdistan Region-Iraq.


Rizgar R. Sulaiman

University of Sulaimania - College of Veterinary Medicine, department of clinic and internal medicine



Abstract
Mycoplasma agalactiae is one of the causal agents of classical contagious agalactia
(CA). It occurs in many parts of the world and most notably in the Mediterranean
Basin. The aims of this study were isolation and molecular identification of
Mycoplasma agalactiae for the first time in Kurdistan region from goats showing
contagious agalactia and in vitro evaluation of the activities of different
antimicrobial against Mycoplasma agalctiae. During the period of January 2011 to
November 2012 a total of 126 milk samples were collected (68 from Slemani
governorate and 58 from Arbil governorate) from goats that had clinical signs of
CA. Mycoplasma agalactiae was recovered from 102(81%) milk samples out of
(126) divided as; 58 (85.3%) out of 68 milk samples in Slemani governorate were
positive for M. agalactiae while 44(75.9%) out of 58 milk samples were positive in
Arbil governorate. All isolates were confirmed by amplification of 1624 bp uvrC
gene by PCR assay. GenBank accession number of the nucleotide and amino acid
sequences of Slemani and Arbil isolates were reported in this study is (KC 594646),
(KC 594647) respectively. The isolates in current work from both governorates
were showed 99% homology to each other and the topology of the phylogenetic
tree indicated that both field isolates were clustered together and they were
belonging to sub linage that contain most of PG2 strains. Danofloxacine and
Azithromycine showed a great effectiveness against all isolates and may be
considered as standard treatment for CA in this region.

Key Words: Contagious agalactia; Mycoplasma agalactiae uvrC gene antibiotic susceptibility



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