Biomass production from Kluyveromyces spp. by using cheese whey

Karzan T. Mahmood

Food Science Dept. Faculty Of Agricultural Science University Of Sulaimani , Kurdistan Region- Iraq

Cheese whey was used as a medium for producing yeast biomass from Kluyveromyces
lactis DSM 70800 and Kluyveromyces fragilis. The best conditions for yeast biomass
production were 35oC with agitation speed of 150 and 200 rpm for Kluyveromyces fragilis
and Kluyveromyces lactis DSM 70800 respectively. Both strains can grow in a wide range
of pH. The maximum biomass yield recorded when whey with pH 6.5 was used. The crud
protein based on dry weight was relatively high and the crud protein % of Kluyveromyces
lactis DSM 70800 (50.56%) was insignificantly higher than that was obtained for
Kluyveromyces fragilis (49.53%) while the protein yield was insignificantly higher for
Kluyveromyces fragilis. On the other hand, the essential and non-essential amino acids
were present in a various amount, among these amino acids arginine was present in high
level in both strains which has the good nutritive value especially for children.

Key Words: Biomass, Yeast, Kluyveromyces, Cheese whey.


[1]. Carrasco de Mendoza M.S, Basílico J.C, Umansky G, and H.E. Scarinci. Biological value of the
unicellular protein of Kluyveromyces marxianus var. lactis. Arch Latinoam Nutr. 41(1): pp 72-8.
[2]. Fadel, M. and M.A. Degheidi. Utilization of whey permeate for yeast production. Egyptian Journal of
Dairy Science. 26: pp 351-362. (1998).
[3]. Genari, A. N., F. V. Passos and F. M. L. Passos. Configuration of a bioreactor for milk lactose hydrolysis.
Journal of Dairy Science. 86: pp 2783-2789. (2003).
[4]. Grba, S., V. Stehlik-Tomas, D. Stanzer, N. Vahèiæ, and A. Škrlin. Selection of Yeast Strain
Kluyveromyces marxianus for Alcohol and Biomass Production on Whey. Chem. Biochem. Eng. Q. 16. 1.
pp. 13–16 (2002).
[5]. Mahmood, Karzan T. Isolation, Characterization and Immobilization of β-galactosidase from the
Yeast Kluyveromyces lactis DSM 70800 and its Application in Some Dairy Products. Ph.D. thesis,
Food science dept. Faculty of agricultural science, Sulaimani university (2010).
[6]. Mahoney, R.R., and Wilder, T. Stabilization of lactase (Escherichia Coli) by milk components and
related compounds. Journal of food science. Vol. 54. Issue 4. pp 899-901. (1989).
[7]. Moeini, H., I. Nahvi and M. Tavassoli Improvement of SCP production and BOD removal of whey with
mixed yeast culture. Electronic Journal of Biotechnology. 7, 3: pp 252-258. (2004).
[8]. Murad, H.A. Abd-el-Ghani, S. and El-shenawy, K. Bioconversion of whey permeate into
Kluyveromyces lactis biomass. Egyptian Journal of Dairy Science. 20: pp 261-271. (1992).
[9]. Popa, A., Mihaela Tapai, and R.C. Duca. Survey on yeast strains biomass production capacity. Physical
and chemical characterisation of the biomass. Archiva Zootechnica vol. 7: pp 81-85. (2004).
[10]. Raimondi, S., Elena Z., Alberto A., Claudio P., Daniela U. and R., Maddalena, Thermal
adaptability of kluyveromyces marxianus in recombinant protein production. Microbial Cell Factories. 12 (34). (2013).
[11]. Rech, R. and M. A. Z. Ayub. Simplified feeding strategies for feed-batch cultivation of
Kluyveromycse marxianus in cheese whey. Process Biochemistry 42: pp 873-877,(2007).
[12]. Shay, L.K. and G.H. Wegner, Nonpolluting conversion of whey permeate to food yeast protein.
Journal of Dairy Science., 69: pp 676-683, (1986).
[13]. Somaye, F., Moosavi-Nasab M. and L., Nasiri, Single Cell Protein (SCP) production from UF
cheese whey by Kluyveromyces marxianus. 18th national congress on food technology. Mashhad I.R. Iran. pp1-6, (2008).
[14]. Szczodark, J. Hydrolysis of lactose in whey permeate by immobilized β-galactosidase from
Kluyveromyces fragilis. Journal of Molecular Catalytalysis B: Enzymatic 10: pp 631-637. (2000).
[15]. Zayed, G.Z. Utilization of whey from reconstituted skim milk from single cell production.
Cultured Dairy Products Journal. 26: 22. (1991).