Purification and Chitinolytic Characteristics of Chitinases A and B from Serratiamarcescens

Jamil A. Baban

Faculty of Medical Sciences, University of Sulaimani, Sulaimaniyha, Kurdistan Region-Iraq
Norwegian University of Life Sciences,P.O. Box 5003, N-1432 Aas, Norway.

DOI: https://doi.org/10.17656/jzs.10531


In Serratiamarcescensthe enzymatic chitinolytic machinery of is one of the best that can convert insoluble polysaccharides.
This process includes four chitin-active enzymes: ChiA, ChiBand ChiC, an endo-acting non-processivechitinase, , two processivechitinases moving along chitin chains in opposite directions. Serratiamarcescensas a Gram-negative soil bacterium produces these three chitinases, ChiA, ChiB and ChiC which together enable Serratiamarcescens to perfectly degrade the insoluble chitin polymer. The data collected for the pseudotrisaccharideallosamidinshows a comparative inhibition including the cyclic pentapeptideargadin. ChiA, ChiBand ChiC play different roles during chitin degradation was confirmed by the synergistic effects that were observed for certain combinations of ChiA, ChiBand ChiC

Key Words: Allosamidin, Chitin,CBP21, Isothermal Titration, Calorimetry, Chitinases, E. coli, harbouring plasmid


[1] Tharanathan RN, KitturFS.,“Chitin-The undisputed biomolecule of great potential”.Crit Rev Food SciNutr Vol. 43, pp 61-87, (2003).

[2] Brurberg MB, Nes IF, Eijsink VGH. “Comparative studies of chitinases A and B from Serratiamarcescens”. Microbiology Vol.142, pp 1581-1589, (1996).

[3] Tews, I., Vincentelli, R., and Vorgias, C. E., “NAcetylglucosaminidase (chitobiase) from Serratiamarcescens: gene sequence, and protein production and purification in Escherichia coli. Gene”, Vol. 170, pp 63–67 (1996).

[4] Suzuki K, Sugawara N, Suzuki M, Uchiyama T, Katouno F, Nikaidou N & Watanabe T. “Chitinases A, B and C1 of Serratiamarcescens 2170 produced by recombinant Escherichia coli: enzymatic properties and synergism on chitin degradation”. BiosciBiotechnolBiochem Vol.66, pp 1075–1083, (2002).

[5] Sakuda, S.; Isogai, A.; Matsumoto, S.; Suzuki, A.; Koseki, K. “The structure of allosamidin, a novel insect chitinase inhibitor, produced by Streptomyces Sp.”. Tetrahedron Lett, Vol.27, No.22, pp 2475-2478, (1986).

[6] Brurberg, M. B., Eijsink, V. G. H., and Nes, I. F., “Characterization of a chitinase gene (chiA) from Serratiamarcescens BJL200 and one-step purification of the gene product”.FEMS Microbiol.Lett., Vol. 124, pp 399–404, (1994).

[7] Brurberg, M. B., Eijsink, V. G. H., Haandrikman, A. J., Venema, G. &Nes, 1. F. “Chitinase B from SerratiamarcescensBJL200 isexported to the periplasm without processing”, Microbiology Vol. 141, pp 123-131, (1995).

[8] Manoil, C., and Beckwith, J., “A genetic approach to analyzing membrane-protein topology”.Science, Vol.233, pp 1403–1408, (1986).

[9] Bjørnar SYNSTAD, Gustav VAAJE-KOLSTAD, F. Henning CEDERKVIST, Silje F. SAUA, Svein J. HORN, Vincent G. H. EIJSINK, and Morten SØRLIE “Expression and Characterization of Endochitinase C from Serratiamarcescens BJL200 and Its Purification by a One-Step General Chitinase
Purification Method”.Biosci.Biotechnol.Biochem., Vol.72, No.3, pp 715–723, (2008).

[10] S. J. HORN, M. SØRLIE, G. VAAJE-KOLSTAD, A. L. NORBERG, B. SYNSTAD1, K. M. VA°RUM, & V. G. H. EIJSINK, “Comparative studies of chitinases A, B and C from SerratiaMarcescens”. Biocatalysis and Biotransformation, Vol.24, No.1/2, pp 39-53 (2006).

[11] AudunSørbotten, Svein J. Horn, Vincent G. H. Eijsink and Kjell M. Va°rum, “Degradation of chitosans with chitinase B from Serratiamarcescens Production of chito-oligosaccharides and insight into enzyme Processivity”.FEBS Journal Vol.272, pp 538–549, (2005).

[12] May BenteBrurberg ,BjørnarSynstad , Sonja SletnerKlemsdal , Daan M.F. van Aalten Leif Sundheim and Vincent G.H. Eijsink. “Chitinases from Serratiamarcescens”.Microbiology.14/12/2000.