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Phytochemical Analysis and Evaluation of Anticonvulsant Effect of Chamomile (Matricaria chamomilla L.) Flower Extract in Chicks


Salam Kamal Seedo & Jian Salam Hassan Ali

Department of Animal Hygiene, Laboratory Researches, Directorate of Veterinary Medicine, Duhok, Kurdistan Region, Iraq.
Department of Pathology and Microbiology, College of Veterinary Medicine, University of Duhok, Kurdistan Region, Iraq.

DOI: https://doi.org/10.17656/jzs.10583

Abstract

This study was carried out to investigate the effect of chamomile flower aqueous extract on different biochemical parameters in pentylenetetrazole (PTZ) induced-convulsive chicks. Parameters included were serum total antioxidant capacity (T-AOC), serum and brain gamma-aminobutyric acid (GABA), serum cholinesterase enzyme activity (ChE), electrolytes (Na+,K+, and Cl-), total calcium (Ca2+), serum glucose and total protein concentration.
The dried flowers parts of chamomile (Matricaria chamomilla L.) were subjected to aqueous extraction and given at (200, 400 and 600mg/kg orally), for its anticonvulsant effects and the effect was compared with the standard anticonvulsant drug sodium valproate (200mg/kg). A dose of 200mg/kg showed full protection against PTZ convulsions, whereas doses of 400 and 600 mg/kg reduced the latency and onset of convulsion. The results suggest that the aqueous extract of chamomile flower may produce anticonvulsant effects via biochemical changes including significant increases in serum T-AOC, serum GABA, serum ChE activity and Na+, and a significant reduction in K+ and serum glucose concentration have been observed.

Key Words:
Matricaria chamomilla L.
PTZ
T-AOC
GABA


References

[1] P. R. Kahn, and P. Lima, "Importation of Matricaria recutita L. (Matricaria chamomilla L.) (German chamomile) as flowers and leaves from EL Salvador, guatemala, hndoras, and nicaragua into the continental united states". U.S.D.A.,Vol. 43, pp. 1-8. (2001).

[2] P.Gardiner, "Complementary, holistic and integrative medicine: chamomile" Pediatr. Rev., Vol. 28, pp.16-8. (2007).

[3] D. L. McKay, and J. B. Blumberg, "A review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.)" Phytother. Res., Vol. 20, pp. 519-30. (2006).

[4] R. Avallone, P. Zanoli, G. Puia, M. Kleinschnitz, P. Schreier, and M. Baraldi, "Pharmacological profile of apigenin, a flavonoid isolated from M. chamomilla L." Biochem. Pharmacol., Vol. 1, pp.1387-1394. (2000). (Abstract).

[5] M. Blumenthal, A. Goldberg, and J. Brinckmann, "Herbal medicine. Expanded commission monographs". Austin. (TX): American Botanical Council, Newton. (MA): Integrative Medicine Communications" pp. 57-61. (2000).

[6] L. Holmquist, C. A. Russo, and A. Elixhauser, "Hospitalizations for epilepsy and convulsions" H.C.U.P Statistical Brief., Vol. 46, pp.1-11. (2005).

[7] A. A. Emmanuel, and O. A. Omolara, "Perception of childhood convulsion among women in a peri-urban community in Ilorin, Nigeria" Journal of Dental and Medical Sciences, Vol. 4m No. 5, pp. 32-38. (2013).

[8] Y. Tanko, E. D. Eze, A. Jimoh, M. Yusuf, K. A. Mohammed, F. Balarabe, M. G. Magaji, and A. Mohammed, "Anticonvulsant activity of methanol stem bark extract of Securinega virosa (Euphobiaceae) in Mice" Journal of Pharmacy and Biological Sciences, Vol. 4, No. 1, pp. 44-47. (2012).

[9] R. K. Malvi, P. Bigoniya, S. Sethi, and S.Jain, "Medicinal plants used in the treatment of epilepsy", approved by International Research Journal of Pharmacy, Vol. 2, No. 2, pp. 32-39. (2011).

[10] D. Nelson, and D.Gray, "Anticonvulsant monitoring in psychiatric practice", Psychiat. Bull., Vol. 25, pp. 356-358. (2001).

[11] F. Robert, and S. Maslah, "Overview of Epilepsy", Stanford Neurology, Comprehensive Epilepsy Center, Stanford, 3th edition, pp. 5-42. (2010).

[12] D.A. Alagpulinsa, "Anticonvulsant and neurobehavioral effects of the aqueous leaf extract of Leea guineensis G. DON (Family: Leeaceae)", M.Sc. Thesis, Faculty of Pharmacy and Pharmaceutical Sciences, University of Science and Technology, Kumasi, (2010).

[13] M. Sushma, M.M. Eswarudu, G. Venkateshwaralu, and P. Radhika, "Evaluation of anti-epilepti cactivity of Psidium guajava leaves extract in Mice" Int. J. of Research in Pharm. and Biom. Sci., Vol. 3, No. 2, pp. 802-806. (2012).

[14] P.K.Samal, "Investigation of antiepileptic activity of Hibiscus vitifolius leaves" Asian J. Pharm. Tech., Vol. 3, No. 2, pp. 63-66. (2013).

[15] M. Mahmoodi, Z. Alireza, and A.Majid, "Anticonvulsant effect of sour orange flowers extract in experimental pentylenetetrazole-induced seizures in rat", Archives of Iranian Medicine, Vol. 6, No. 3, pp. 212-213. (2003).

[16] A.H. Yaro, J.A. Anuka, O.A. Salawu, and M.G. Magaji, "Anticonvulsant activities of methanol extract of Chrysanthellum indicum Linn. Vatke in mice and chicks" Nigerian Journal of Pharmaceutical Sciences, Vol. 6, No. 2, pp. 22-27. (2007).

[17] Y.J. Mousa, "Effect of chlorpheniramine on acute dichlorvos poisoning in chicks" Iraqi Journal of Veterinary Sciences, Vol. 23, No. 2, pp. 35-43. (2009).

[18] M.S. Shabbir, A. Arfan, M.N. Zahid, N. Jawad, N. Muhamamd, and A. Haroon, "Sample collection guide, a practical approach" Nexus Academic Publishers, pp. 1-6. (2013).

[19] B.K. Al-Baggou, A.S. Naser, and F.K. Mohammad, "Hydrogen peroxide potentiates organophosphate toxicosis in chicks" Human and Veterinary Medicine International Journal of Bioflux Society, Vol. 3, No. 2, pp. 142-149. (2011).

[20] F.K.Mohammad, "Review of a practical electrometric method for determination of blood and tissue cholinesterase activities in animals" Vet. Scan, Vol. 2, No. 2, pp. 1-12. (2007).

[21] P.Trinder, "Rapid method for the determination of sodium in serum" Analyst, Vol. 76, pp. 596-599. (1951).

[22] G. Hillmann, and G. Beyer, "Turbidimetric determination of potassium in serum" Z. Klin. Chem. Klin. Biochem., Vol. 5, No. 2, pp. 92-93. (1967).

[23] D.M. Zall, D. Fisher, and M.Q.Garner, "Photometric determination of chloride in water" Analytical Chemistry, Vol. 28, pp.1665-1668. (1956).

[24] W.R. Moorhead, and H.G. Briggs, "2-Amino-2-methyl-1-propanol as the Alkalizing Agent in an Improved Continuous-Flow Cresolphthalein Complexone Procedure for Calcium in Serum" Clinical Chemistry, Vol. 20, No. 11, pp.1458-1460. (1974).

[25] P. Trinder, "Determination of glucose in blood using glucose oxide with an alternative oxygen acceptor" Ann. Clin. Biochem., Vol. 6, pp. 24-27. (1969).

[26] A. C. Gornal, C. J. Bardawill, and M. M. David, "Determination of serum proteins by means of the biuret reaction" J. Biol. Chem., Vol. 177, No. 2, pp.751-756. (1949).

[27] V.H.A. Enemor, "The effects of ethanol extract of roots of Sarcocephalus latifolius on some biochemical parameters in wistar albino rats and it is antimalarial potentials",Ph.D. Thesis, Faculty of Bioscience, University of Nnamdi Azikiwe, Nigeria, (2012) .

[28] N. M. Danjuma, H.T. Saulawa, A. U. Zezi, A. H. Yaro, A. Giwa, and M.G. Magaji, "Anticonvulsant potential of the stem bark extract of Acacia albida", International journal of Applied Research in Natural products, pp. 40-46. (2009).

[29] D. Sudipta, K. H. Pallab, P. Goutam, P. P. Siva, and B. Samit, "Anticonvulsant activity of methanolic extract of Clerodendronin fortunatum Linn. In Swiss albino mice", Thai. J. Pharm. Sci., Vol. 34, pp. 129-133. (2010).

[30] P.Amoateng, "Anticonvulsant and related neuropharmacological effects of a hydro-ethanolic whole plant extract of Synedrella nodiflora L. Gaertn" Ph.D. Thesis, Faculty of Pharmacy and Pharmaceutical Sciences, University of Science and Technology, Kumasi, (2011).

[31] M. G. Magaji, A. H. Yaro, A. M. Musa, J. A. Anuka, I. Abdu-Aguye, and I. M. Hussaini, "Neuropharmacological studies on ethyl acetate fraction of Securinega virosa root bark extract" African Journal of Pharmacy and Pharmacology, Vol. 7, No. 6, pp. 275-279. (2012).

[32] M. Shyamjith, B. Deepa, E. J. Anu, and S. N. Rao, "Anticonvulsant activity of Phyllanthu samarus in experimental animal models" International Journal of Applied Biology and Pharmaceutical Technology, Vol. 2, pp. 144-149. (2011).

[33] M. Raza, F. Shaheen, M. I. Choudhary, S. Sombati, A. Rafiq, A. Suria, A. Rahman, and R. J. DeLorenzo, "Anticonvulsant activities of ethanolic extract and aqueous fraction isolated from Delphinium denudatum" J. Ethnopharmacol., Vol. 78, No. 1, pp. 73-78. (2001).

[34] J. S. Duncan, "The promise of new antiepileptic drugs" B. R .J. Clinical Pharmacology, Vol. 53, No. 2, pp.123-131. (2002).

[35] N. A. K. Hamid, "The effect of antioxidant supplementation in the treatment of epilepsy" Irq. J. Pharm., Vol. 11, No. 2, pp. 27-33. (2011).

[36] M. Bindu, R. Krishnan, and V. K. Rajendiran, "Low plasma antioxidant status in patients with epilepsy and the role of antiepileptic drugs on oxidative stress" Ann. Indian Acad. Neurol., Vol. 17, No. 4, pp. 398-404. (2014).

[37] H. Karunakar, P.T. Shalin, B. J. Arun, C. S. Shastry, and K. S. Chandrashekhar, "Anticonvulsant Activity of Carissa carandas Linn. root extract in experimental mice" Tropical Journal of Pharmaceutical Research, Vol. 8, No. 2, pp. 117-125. (2009).

[38] K. Cui, X. Luo, K. Xu, and M. R. Ven Murthy, "Role of oxidative stress in neurodegeneration, recent developments in assay methods for oxidative stress and nutraceutical antioxidants" Progress in Neuro-Psychopharmacology & Biological psychiatry, Vol. 28, pp. 771-799. (2004).

[39] T. Makhmoor, "Antioxidant from medicinal plants and of synthetic origin", Ph.D. thesis, International Center of Chemical Science, University of Karachi, Pakistan, (2005).

[40] M. A.Moetazza, S. K. Seham, and M. A. Madeha, "Effect of long term treatment with antiepileptic drugs on oxidant status, zinc and magnesium in epileptic patients" World Applied Sciences Journal, Vol. 28, No. 3, pp.316-323. (2013).

[41] S. A. Hamed, M. M. Abdellah, and N. El-Melegy, "Blood levels of trace elements, electrolytes, and oxidative stress/antioxidant systems in epileptic patients" J. of Pharm. Sci., Vol. 96, pp. 465-473. (2004).

[42] K. H. Schulpis, C. Lazaropoulou, S. Regoutas, G. A. Karikas, A. Margeli, S.Tsakiris, and I. Papassotiriou, "Valproic acid monotherapy induces DNA oxidative damage" Toxicology, Vol. 217, No. (2-3), pp. 228-232. (2006), (Abstract).

[43] E. Plonka-Poltorak, P. J. Zagrodzki, H. Chlopicka, T. Barto , P. Westermarck, M. Kaipainen, Kaski and F. Atroshi, "Valproic acid modulates superoxide dismutase, uric acid-independent and zinc in blood of adult epileptic patients" Biol. Trace Elem. Res., Vol. 143, pp. 1424-1434. (2011) (Abstract).

[44] Z. Zhang, X. Qin, X.Zhao, N.Tong, Y. Gong, W.Zhang, and X.Wu, "Valproic acid regulates antioxidant enzymes and prevents ischemia/reperfusion injury in the rat retina" Curr. Eye Res., Vol. 37, pp. 429-437. (2012).

[45] C.Wasowski, and M. Marder, "Flavonoids as GABAA receptor ligands: the whole story" Journal of Experimental Pharmacology, Vol. 4, pp. 9-24. (2012).

[46] P. Gardiner, "Chamomile (Matricaria recutita, Anthemisnobilis)" Longwood Herbal Task Force. pp. 1-21. (1999). Available at http://www.mcp.edu/herbal/default.htm. Jan. 2015.

[47] L. Zaiter, M. Bouheroum, S. Benayache, F. Benayache, F. Leo´n, I. Brouard, J. Quintana, F. Este´vez, and J. Bermejo, "Sesquiterpene lactones and other constituents from (Matricaria chamomilla L.)" Biochemical systematic and ecology, Vol. 35, pp. 533-538. (2007).

[48] O. P Mishra, D. Singhala, R. S. Upadhyayb, R. Parasada, and D. Atria, "Cerebrospinal fluid zinc, magnesium, copper and gamma amino butyric acid levels in febrile seizures" J. of Pediatric Neu., Vol. 5, pp.39-44. (2007).

[49] O. N. Salah, E. R. Abdelraouf, M. H. Abdelhameed, A. A. Dawood, A. F. Hashish, A. Kilany, and S. I. Helal, "Assessment of the level of GABA and some trace elements in blood in children who suffer from familial febrile convulsions" Macedonian J. of Medical Sci., Vol. 7, No. 1, pp. 68-73. (2014).

[50] D. Jack, "Principles of drug action GABA-minergic neurotransmission introduction" Vol. 2, pp. 1-27.(2004).

[51] P. Scholze, V. Ebert, and W. Sieghart, "Affinity of various ligands for GABA-A receptors containing alpha 4 beta 3 gamma 2, alpha 4 gamma 2, or alpha 1 beta 3 gamma 2 subunits" Eur. J. Pharmacol., Vol. 304, No. (1-3), pp. 155-162. (1996).

[52] A. D. Abdel Mageid, A. A. Al- Komi, and I. M. Abdel Wadoud, "Biochemical effect of L-Carnitine in valporate induced hyperammonemia in rabbits" Benha Veterinary Medical Journal, Vol. 27, No. 2, pp.197-208. (2014).

[53] C. J. Landmark, "Targets for antiepileptic drugs in the synapse" Med. Sci. Monit., Vol. 13, pp. 1-7. (2007).

[54] J.S.H. Ali, L.I. Matty, and S.S.Yahya, " Effect of induced epilepsy on some biochemical parameters in female rats" Iraqi Journal of Veterinary Sciences, Vol. 26, No. 2, pp. 89-92. (2012).

[55] G. M. Cunha, G. FaViana, and P. A. Srais, "Evidence for the involvement of the muscarinic cholinergic system in the central action of pentoxifylline" Behave. Pharmacol., Vol. 13, No. 2, pp.149-156. (2012).

[56] K. S. A. Askar, "Characterization of cholinesterase activities for pesticide exposure in food animals" Ph.D. Thesis, Faculty of Science and Technology, University of Plymouth, U.K, (2012).

[57] M. J. Tutor-Crespo, J. Hermida, and T. J. Carlos, "Possible induction of cholinesterase in epileptic patients treated with anticonvulsant drugs, relationship with lipoprotein levels" J. Clin. Pharmacol., Vol. 44, pp. 974-980. (2004).

[58] D. Gamit, S. Hariom, C. Nitinkumar, M. Nikunj, and K. Gamit, "Liver enzymes activity during sodium valproate therapy in patient with epilepsy" Int. J. Res. Med., Vol. 2, No. 2, pp. 30-33. (2013).

[59] A. K. Sawhney, and J. A. Lott, "Clinical Enzymology. Acetyleholinestrase and cholinesterase in: Lott, J. A. and Wolf, P. L" New York, Field, Rich and Associates, pp. 1-26. (1986).

[60] W. G. Hozayen, R. R. Ahmed, A. K. Khalefa, and M. B. Ahmed, "Modulatory effects of fish liver oil on pilocarpine-model of epilepsy in Rats compared to topirmate as a common antiepileptic drug" Journal of American Science, Vol. 4, pp. 559-566. (2012).

[61] H. H. Abdul Wahid, "Evaluation of serum electrolytes and uric acid in Iraqi epileptic patients" Iraq Postgraduate Medical Journal, Vol. 9, No. 1, pp. 84-87. (2010).

[62] D. A. Nzeh, R. T. Erasmus, and B. A. Aiyedun, "Serum electrolytes and protein changes after intravenous injection of sodium and megluminediatrizoate" Afr. J. Med. Sci., Vol. 23, pp.35–37. (1994).

[63] R. Carmen, R. Moises, R. Socorro, L. Mariso, C. Verónica, and P. Carlos, "In vivo experimental models of epilepsy" Central Nervous System Agents in Medicinal Chemistry, Vol. 10, No. 4, pp. 298-309. (2010).

[64] P. Kwan, G. J. Sills, and M. J. Brodie, "The mechanisms of action of commonly used antiepileptic drugs" Pharmacol. Therapy, Vol. 90, No. 1, pp. 21-34. (2001).

[65] H. S. Waagepetersen, and A. Schousboe, "GABA neurotransmission an overview" Springer Science‏ Media, Vol. 9, pp. 213-222. (2008).

[66] S. Kumar, V. Kumar, M. Reena, and D. C. Jain, "Trace Elemental Analysis in Epileptic Children" Open Journal of Applied Sciences, Vol. 3, pp. 449-476. (2013).

[67] J. S. H. Ali, "Effect of epilepsy and antiepileptic drugs on biochemical parameters in human and experimental rats" Ph.D. Thesis, Faculty of Veterinary Medicine, Duhok University, Iraq. (2010).

[68] A. Yuzo, S. Masayuki, H. Katuya, and S. Tadao, "Haematological and serum biochemical values in spontaneously epileptic male rats and related rat strains" Laboratory Animals, Vol. 32, pp.214-218. (1998).

[69] V. Pylvanen, "Insulin-related metabolic and endocrine effects of valproate in patients with epilepsy" Master Thesis, Faculty of Medicine, Department of Neurology and Clinical Chemistry, University of Oulu, Finland. (2005).

[70] D. F. J. Connor, "Severe acute necrotising pancreatitis caused by sodium valproate" Critical Care and Resuscitation, Vol. 1, pp.366-367. (1999).

[71] H.Okayasu, T.Shinozaki, A. Osone, Y. Ozeki, and K. Shimoda, "Development of acute pancreatitis caused by sodium valproate in a patient with bipolar disorder on hemodialysis for chronic renal failure" Bio. Med. Central. Psychiatry, Vol. 14, pp. 93-96. (2014).

[72] S. J Bhathena, and M. T. Velasquez, "Beneficial role of dietary phytoestrogens in obesity and diabetes" American Journal of Clinical Nutrition, Vol. 76, No. 6, pp. 1191-1201. (2002)..

[73] J. D. Wagner, W. T. Cefalu, M.S. Anthony, K. N. Litwak, L.Zhang, and T. B. Clarleson, "Dietary soy protein and estrogen replacement therapy improve cardiovascular risk factors and decrease aortic cholesterol" Metabolism, Vol. 6, No. 46, pp. 698-705. (1997).

[74] S. Natelson, D. J. Miletich, C. F. Seals, D. J. Visintine, and R. F. Albrech, "Clinical biochemistry of epilepsy" Clin. Chem., Vol. 24, No. 6, pp. 889-897. (1979).

[75] P. Garzon, S. Gonzalez-Cornejo, S. Roman-Maldonado, and A. Navarro-Ruiz, "Valproic acid and phenytoin effects on serum proteins and immunoglobulins of epileptic patients" Gen. Pharmace., Vol. 16, No. 4, pp. 411-413. (1985).

[76] A. Babayigit, E. Dirik, E. Bober, and H. Cakmakci, "Adverse effect of antiepileptic drugs on bone mineral density" Pediatric Neurology, Vol. 35, No. 3, pp.177-181. (2006).

[77] M. Raza, A. Alghasham, M. S. Alorainy, and T. M. El-Hadiyah, "Beneficial interaction of thymoquinone and sodium valproate in experimental models of epilepsy Reduction in hepatotoxicity of valproate" Scientia. Pharmaceutica, Vol. 74, pp.159-173. (2006).