Determination of tetracycline hydrochloride in some pharmaceutical products using flow injection spectrophotometric techniquel

Shirwan Omer baban1 , Rasul Jameel Ali2

1 Chemistry Department, College of Education Science, Salahaddin University-Hawler, Iraqi-Kurdistan Region 2 Ministry of
Agriculture and Water Resources, Erbil
2 Clinical Biochemistry department, College of Health Sciences, Hawler Medical University, Hawler / Iraqi-Kurdistan Region

Received: 11 August. 2015;  Accepted: 30 August. 2015;  Published Online: 20 March. 2016

DOI Link: 


A simple, rapid, and sensitive flow injection spectrophotometric system for determination of tetracycline hydrochloride (TCH) in pharmaceutical products. The method is based on diazotization of 2-aminopyridine and subsequent coupling with tetracycline hydrochloride in alkaline medium. The formed azo-dye showed maximum absorbance at 388 nm and the calibration curve in the linear range of 25.0-150.0 μg/ml of tetracycline hydrochloride with correlation coefficient 0.9988, while the detection limit was 10 μg/ml and sample frequency 123 samples/hour. The accuracy and precision data for determination of TCH by flow injection analysis method for triplicate injection at three concentration levels(25, 80 and 150 μg/ml) the relative standard deviation (RSD %) are 1.04, 0.247 and 1.75%, and relative error percent (Erel) are - 3.92, +1.06 and -1.9%. The proposed method was successfully applied to the determination of tetracycline hydrochloride in pharmaceutical products. The results obtained were in good agreement with those obtained by reference method.


Tetracycline hydrochloride; 2-aminopyridine; flow injection spectrophotometric



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