jzs-10639

Prevention of Adhesion Following Hysterotomy Using Omentum in Rabbits

Talib Gaidan Minat Ali, Sozan Ali Muhamad and Mardin Omer Jola

Department of Surgery and Theriogenology, College of Veterinary Medicine, Sulaimani University, Kurdistan Region, Republic of Iraq.


Abstract

Adhesion formation is a major source of the postoperative threaten the life of the animals. The purpose of the investigation is the effect of autologous graft by using the omentum to reduce and prevention of adhesion throughout hysterotomy in rabbits. Study was applied on 20 local breed rabbits that divided randomly into 2 groups of 10 animals each. Aseptic surgical laparotomy and hysterotomy done for all rabbits, and the omentum were used in treatment group. Postmortem and microscopic examination showed the presence of mild to severe adhesion in control group. At the 14th postoperative days, the degrees of adhesions were graded as: grade (1) in two rabbits, and (2) in the remaining two rabbits. Also at 28th post operative days, different adhesions formed and were graded as: grade (1) in two rabbits, grade 1 and 3 in one rabbit each. Conversely, in treatment group, at 14th postoperative day, 4 rabbits shown adhesion grade (2) in each 2 rabbits. While at 28th postoperative day, grade 1 adhesion was found in 3 rabbits that exhibiting moderate band of adhesion. The results of 2 run time referred that adhesion extension increased in control group with the time. The average p- value for adhesion of extension in all rabbits of treatment group was 1.50±0.577 and 1.25±0.500 at day 14th and 28th respectively. Histological examination revealed significantly high score of fibrosis with infiltration of inflammatory cells especially in control group, with slightly infiltration of inflammatory cells within muscular and serosal layers in treatment group. In conclusion, we confirmed that the using of autologous graft especially the omentum leads to somewhat reduction and prevention of adhesion during postoperative hysterotomy in rabbit model

KeywordsAdhesion, Autologous graft, Hysterotomy, Rabbit
References: 

[1] Amelia P Bailey, Amy K Schutt2, Lisa M Pastore and Dale W Stovall2, "Pelvic pedicled omental flaps and autologous free omental grafts in a rabbit model. Bailey et al". Fertility Research and Practice 2015, 1:3 http://www.fertilityresearchandpractice.com/content/1/1/3. ( 2015).

[2] Chung, D.R.; Chitinis, T.; Panzo, J.R.; Kasper, L.D.; Sayegh, H.M. and Tzianabos O.A. "CD4 +T cells Regulate surgical and post infectious adhesion formation". Journal Experimental Medicine, Vol. 195, No. 11m pp. 1471-1478. (2002).

[3] Claudio, B., Fabrizo, M., Daniele, P., Alessandra, P., Emilio, B., Massimo, D., Marco, F., Donato, N. and Mario, Lise. "Prevention of postsurgical adhesions with an autocrosslinked hyalonan derivive gel". J. Surg. Res, Vol. 100, pp. 217-221. (2001).

[4] Fatih Mehmet Avasar,Mustafa Sahin, Faruk Akosy, A.Filiz Avasr, "effect of Diphenelhydramine HCL and Methylpredinsolone in the prevention of abdominal adhesions". The American Journal of Surgery.Vol. 181, pp. 512-515. (2001).

[5] Gul, A.Kotan., Sahin, G; Eryavuzy, "The effectiveness of intrapretonial usage of hydroxyprogesterone ccaproat in prevention of postoperative adhesion; An expemental study". Estern Journal Medicine. Vol. 4, No. 2, pp. 77-79. (1999).

[6] Hall, L.W., Clark, K.W., and Trim, C.M. "Veterinary anesthesia". 9th  Chapter,17:W.B.Sauders company Ltd. (2003).

[7] Moll, D.H.; Schumacher, J.; Wright, C.J.; and Spano, S.J. "Evaluation of sodium carboxymethyle cellulose for prevention of experimentally induced abdominal adhesion in ponies". American Journal Veterinary Research, Vol. 52, pp. 738-741. (1991).

[8] Oktay, I., Zafer, F., Lelya. M.,and Ozgur, E. "Reduction of postsurgical adhesion in a rabit model". Acoparative study,clinics, Vol. 64, No. 2, pp. 7-9. (2009).

[9] Singer, E.R.; Livesey, MA; Barker, I.K.; Hurtig, M.B.; and Conlon, P.D. "Utilization of the serosal scarification model of postoperative intestinal adhesion formation to investigate potential adhesion preventing substances in the rabbits". Canadian Journal Veterinary Research, Vol. 60. Pp. 305-311. (1996).

[10] Thornton,M.and dizerega, G.S. "The use of barriers in adhesion prevention". Prevention. Contemp.Obstet.Gyneco. Vol. 41, pp. 201-214. (1996).

[11] Toosie, K.; Gallego , K.; Stabiley , B.; Schaber, B.; French, S.; and Virgilo, C. "Fibrin glue reduce intra-abdominal adhesions to synthetic mesh in a rat ventral hernia model". American Surgery, Vol. 66, pp. 41-45. (2000).

[12] Topdag, M., Kara, A., Konuk, E., Demir, N., Ozturk, M., Calisk, S., Topdag, D.O., Ulubi, A., Kskin, I.G. and Iseri, M. "The healing effect of autologous mucosal grafts in experimental injured rabbits maxillary sinusis". Clinical and experimental otorhinolaryngology. Vol. 9, No.1, pp. 44-50. (2016).

[13] Verco, J.S.; Peers, M.E.; Brown, B.C.; Rodgers, E.K.; Roda, N.; and diZerega, G. "Development of a novel glucose polymer solution (icodextrin) for adhesion prevention. Pre-clinical studies". Human Reproduction, Vol. 15, No. 8. pp. 1764-1772. (2000).

[14] William, W.M. and John, A.E. "Handbook of Veterinary", Anaesthesia 2nd Ed., pp. 366-367. (1995).

[15] Yoon, y. and Kohane, D.S. "Polymers in the prevention of peritoneal adhesion". Eur. J. Pharm. Biopharm. Vol. 68, No. 1, pp. 57-66. (2008).