Batch and Flow injection Spectrophotometric Methods For Determination of Folic acid in Pharmaceutical Preparations using Cerium(IV) ammonium sulphate(CAS) as an oxidant.

Shirwan Omer Baban1 & Kurdistan F. Azeez1

1 Department of Chemistry, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq.

Original: 29 May 2017, Revised: 7 May 2018, Accepted: 11 June 2018, Published online: 20 June 2018


A simple, rapid and sensitive batch and flow injection spectrophotometric methods have been developed for the determination of folic acid (FA) in pure form and in its pharmaceutical preparations. The proposed methods involve the addition of a measured excess of cerium (IV) ammonium sulphate (CAS) in acidic medium followed by determination of unreacted CAS by reacting with a fixed amount of methylene blue (MB) and measuring the absorbance at 665 nm. The optimum reaction conditions and other analytical parameters have been evaluated. Linearity was observed from 1-10 and 4.0-20.0 μg/ml folic acid by batch and flow injection procedures, respectively. Statistical analysis of the results and comparison with results by the British Pharmacopoeia method are also reported.

Key Words: Spectrophotometric Folic acid, Flow-injection, Methylene blue Cerium (IV) ammonium sulphate


[1] P.S. Ganesh and K.S. BE, "Poly (Patton and Recederas) Modified Carbon Paste Electrode Sensor for Folic Acid", Biosensors and Bioelectronics, (2016).

[2] K.K. Hammud, J.M. Mohammed, M.M. Radif, A.L.M. Raouf , S.S. Mahmmood, R.R. Neama, D.E. Znad and S.J. Abbas, ''Thermometric Parameters for Phenanthrene Interaction with a Biological II-Acceptor (Folic Acid) by Spectroscopic Measurements'', J. Chemical and Pharmaceutical Research, Vol. 5, No. 2, pp. 24-28. (2013).

[3] S. Esfandiarpour-Boroujeni, S. Bagheri-Khoulenjani and H. Mirzadeh, "Modeling and Optimization of Degree of Folate Grafted on Chitosan and Carboxymethyl-Chitosan". Progress in biomaterials, Vol. 5, No. 1, pp. 1-8. (2016).

[4] V. Sreeram, M.V. Basaveswara and S.R. Karumuri, ''A Validated and Stability Indicating Ultra High Pressure Liquid Chromatographic Method for Determination of Folic Acid in Pharmaceutical Preparations'', International Journal of Chemical Studies, Vol. 1, No. 1, pp. 17-27.(2013).

[5] E.E. Abano, and R.G. Dadzie, ''Simultaneous Detection of Water-Soluble Vitamins Using the High Performance Liquid Chromatography (HPLC)- a review''. Croatian J. of Food Science and Tecnology, Vol. 6, No. 2, pp. 116-123. (2014).

[6] R. Rengarajan, H.M. Albishri and D.A. El-Hady, "Eco-friendly and Rapid Ultra High Pressure Liquid Chromatography for Simultaneous Determination of Methotrexate and Folic Acid in Human Urine'', American Journal of Modern Chromatography, Vol. 1, No. 2, pp. 55-66. (2014).

[7] J. Ghasemi, B. Abbasi, A. Niazi, E. Nadaf and A. Moradi "Simultaneous Spectrophotometric Multicoponent Determination of Folic Acid, Thiamine, Riboflavin, and Pyridoxal by Using Double Divisor-Ratio Spectra Derivative-Zerp Crossing Mehod", Analytical letters, Vol. 37, No. 12, pp. 2609- 2623. (2004).

[8] W. Ruengsitagoon and N. Hattanat, "the 4rth Annual Northeast Pharmacy Research Conference of 2012", Pharmacy Profession in Harmony.

[9] A.N. Jasim, "New Approach for the On-line Spectrophotometric Determination of Folic Acid in Pure and Pharmaceutical Preparation via Oxidation by Cerium (IV) Sulphate Using Ayah 3Sx3-3D-Solar Cell CFI Spectrophotometer Analyzer"', Iraqi Journal of Science, Vol. 55, No.3B, pp. 1153-1163. (2014).

[10] N.S. Othman, S.H. Hasan and K.M. Surchi, "Indirect Spectrophotometric determination of FA Based on the Oxidation reaction Thermodynamic parameters", J.Zankoi Sulaimany, Vol. 17, No. 1, pp. 61-70, (2015).

[11] M. Khateeb, B. Elias and F.A. Rahal, "New Kinetic Spectrophotometric Method for Determination of Folic Acid in Pharmaceutical Formulations"', Internal Latters of Chemistry,physics and Astronomy, Vol. 50, pp. 169-178. (2015).

[12] S.M. Wabiadur, S.M. Alam, S.H. Lee, Z.A. Alothman and G.E. Eldesoky, "Chemiluminescence Determination of Folic Acid by a flow injection analysis assembly",  pectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 105, pp. 412-417. (2013