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Biochemical and molecular identification with antimicrobial susceptibility of bacterial species isolated from organs and tissues of Alectoris chukar subspecies Kurdistanica

Heshu Sulaiman Rahman1,2*, Kwestan Najm Ali3, Nawzad Rashid Abdulrahman3, Peshnyar Muhammad Atta Rashid4, Alan Ahmed Mahmood2, Hemn Hassan Othman5, Kawa Amin1, Rasedee Abdullah6


1College of Medicine, University of Sulaimani, Sulaimani, Kurdistan Region- Iraq

2College of Health Sciences, Komar University of Science and Technology, Chaq-Chaq Qularaesee, Sarchinar District, Sulaimani, Kurdistan Region- Iraq

3College of Veterinary Medicine, University of Sulaimani, Sulaimani, Kurdistan Region- Iraq

 4Molecular Diagnostic Laboratory, Sulaimani Veterinary Directorate, Sulaimani, Kurdistan Region- Iraq

5College of Pharmacy, University of Sulaimani, Kurdistan Region- Iraq

6Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Original: 20 January 2020       Revised: 10 March 2020     Accepted:April 2020        Published online: 20 June 2020  

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The current study was conducted on 50 Alectoris chukar subspecies Kurdestanica that was collected from Sulaymaniyah Province, Kurdistan Region, Northern Iraq, during the period of April to the end of September 2016. Samples of liver, gallbladder, spleen, kidneys, heart, lungs, gizzard, breast, and thigh muscle tissues were tested for bacterial isolates. Preliminary characterization of the isolated bacteria was carried out by morphological and biochemical methods. The VITEK 2® system was used to confirm the isolated species, while the polymerase chain reaction (PCR) was used for the detection of the resistance gene in the bacterial isolates. As a result, tested samples showed the presence of Staphylococcus sciuri and Escherichia coli. Additionally, antimicrobial susceptibility test was done to determine the bacterial susceptibility to various antibiotics and as a result; E. coli showed 100% susceptibility to penicillin, azithromycin, tetracycline, and doxycycline and 75% susceptibility to streptomycin. On the other hand, S. sciuri exhibited 75% susceptibility to azithromycin, penicillin, and doxycycline, 50% susceptibility to streptomycin, and 25% susceptibility to tetracycline. Molecular identification showed that only the S. sciuri isolates carried the methicillin-resistant mecA gene. To our knowledge, this is the first record of isolation of the S. sciuri methicillin-resistant mecA gene from A. chukar subspecies Kurdestanica.

Key Words: Bacterial isolates, wild bird, biochemical test, VITEK 2 system, antibiotic susceptibility, gene sequencing


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