The Antibacterial activity of Pistacia Khinjuk resionous exudates

Bahrouz M. A. Al-jaff

College of Science, University of Sulaimani

A crude resinous exudate from Pistacia lentiscus related Pistacia khinjuk tree distributed in Kurdistan mountains has been used focally in curing some lesions including burns. The preparation solved in 50% ethanol and diluted by distilled water was appeared to have antibacterial activity. The minimum inhibitory concentration (MIC) of the resin by well diffusion agar for Staphylococcus aureus was 50 pg/ml and with no effect on P. aeruginosa. By dilution broth method, MIC was 125 pg/ml for S. aureus and 500 pg/ml for P. aeruginosa .The killing effect against P. aeruginosa occurs also by the solvent 50% ethanol which revealed that resin has no effect on this species. The minimum bactericidal concentration was the same of MIC for both species. The effect of resin was not belonged to phenol only that the MIC of phenol by well diffusion agar was lower than resin for S. aureus. 
Keywords: Pistacia khinjuk, Pistacia lentiscus, mastic, resin, antibacterial activity, bacteria, Staphylococcus aureus, Pseudomonas aeruginosa. 

[I ]Towsened, C. C. and Guest, E. Flora of Iraq. Part 1, Cornaceae to Rubiaceae. 1980 , 4,Ministry of Agriculture and Agrarian reform, Baghdad, Iraq. 
[2]Galen. Galen; Selected works. Oxford University Press.: 1996, 448.
[3]Abdul-Rahman, A. Mastiche as an antioxidant. J. Am. Oil chem. Soc, 1975, 52, 423. 
[4] Villar, A., Sanz, M. J. and Paya, M. Hypotensive effect of Pistacia lentiscus L. Intl. Crude Drug Res. 1987 , 25(1) 1-3. 
[5] Al-Said, M. S., Ageel, A. M., Paramar, N. S. and Tariq, M. Evaluation of mastic, a crude drug obtained from Pistacia lentiscus for gastric and duodenal anti-ulcer activity. J. Ethnopharmacol. 1986,15,271 — 278. 
[6] Keys, J. D. Chinese herbs, their botan, chemistry and pharmacodynamics. 1976. Charles E. Tuttle company, Rutland VT, Tokyo. 
[7]Al-Habbal, M. J., Al-Habbal, Z. and Huwez, F. W. A double-blind trail of mastic (Saladin) and placebo in treatment of duodenal ulcer. Proceedings of the Third International ConliT ewe on Islamic Medicine, Istanbul, Turkey. 1984,105.
[8] lavk,, L. ,Ragusa S. Rapisarda A. , Tranco . S. and Nicolosi , V. M. I In vitro antimicrbial activity of Pistacia lentiscus L. extracts: Preliminary report. J. Chemotherapy, 1996 ,8(3), 207-209. 
[91 Magiastis„ P,„ Mellion, E., Skaltsounis, A-L., Chinou, I.B. and Mitaku, S. Chemical composition and antimacrobial activity of essential oils of Pistacia lentiscus var. chin. Plant Med., 1999, 65, 749-752.
[10] Ali-Siltayeh, M.S. and Abu-Ghadeib, S. I. Antifungal activity of plant extracts against dermatophytes. Mycosis, 1999,42, 665-672.
[11] Garrod, H. B. and Heatly, N. G. Bacteriological methods in connection with penicillin treatment. Br. .I. Surg. 1944, 32, 117-124. 
[12] National committee for clinical laboratory standards for antimicrobial susceptibility testing. 1992. Villanova, Pa. 
[13] Yoshida, S., Kasuga, S., Hayashi, N., Ushiroguchi, T., Matsuura, H., and Nakagawa, S. Antifungal activity of ajoene derived from garlic. Appl. Environ. Microbiol. 1987, 53(3), 615-617.