Incidence of Rose Bengal Positive Agglutination Test Among Blood Donors in Sulaimani Blood Bank

Sherko A. Omer

College of Medicine, University of Sulaimani

This study was achieved to determine Brucella antibodies using Rose Bengal agglutination test in serum of healthy persons attending a blood bank in Sulaimani city in Iraqi Kurdistan region, each person's serum was tested directly without dilution and with 1:80 dilution, the overall positive agglutination results were observed in 29 out of 548 cases (5.29%). Twenty seven of them gave negative reaction with 1:80 dilution, we recommend that if only Rose Bengal test is used to confirm diagnosis of brucellosis, titration of patients serum is useful for better evaluation.

Keywords: - Brucellosis, Agglutination, Rose Bengal test, blood donors


[1] Mark H. Beer and Robert E. The Merck manual, Merck research laboratories, 2000,0911910-10-7 , pp: 1166-1167
[2] Lulu AR et al. Brucellosis in Kuwait: a prospective study of 400 cases. Quarterly journal of medicine, 1988, 66(249):39-54
[3] WHO. Brucellosis surveillance report 1988. In: Proceedings ofthe First International Conference on Brucellosis. Mosul, Iraq, University of Mosul, 1990:48.
[4] Mohammed Ali R. Y., Baghdasarian Z.S. Brucellosis in Ninevah Province. In: Proceedings ofthe First International Conference on Brucellosis. 1990:21. Mosul, Iraq, University of Mosul,
[5] Joint FAO/WHO Expert Committee on Brucellosis: [meeting held in Geneva from 12 to 19 November 1985] sixth report. Geneva, World Health Organization, 1986. (WHO Technical Report Series 740). {6] Gazapo E. etal. Changes in IgM and IgG antibody concentrations in brucellosis over time: importance for diagnosis and follow-up. Journal of infectious diseases, 1989,  225.
[7] RuseeI ,A.O, C.M. Patton , and A.F. Kaufman . Evaluation of the card test for diagnosis of human brucellosis ,J.CIin Microbiol. . 1978, 7:454-458.
[8] W .A. Dabdoob and Z.A. Abdulla. A panel of eight tests in the serodiagnosis and immunological evaluation of acute brucellosis. Eastern Mediterranean Health Journal. , 6 , 23
[9] Ma1tin-Moreno-S; Guinea-Esquerdo-L; Carrero-Gonzalez-P; Visedo-Orden-R; GarciaCarbajosa-S; Calvo-deI-Olmo-T; Reverte-Cejudo-D.Diagnosis of blucellosis in an endemic area. Evaluation of routine diagnostic tests.Med-CIin-Barc. 1992 , 98(12): 481-5 [10]Colmenero-JD; Reguera-JM; Cabrera-FP; Hernandez-S; Pon•as-J; Manchado-P; MirandaMT .Combined use of rose Bengal and indirect immunofluorescence in the diagnosis of brucellosis: Enferm-Infecc-Microbiol-CIin. 1989, 7(6): 316-20.
[11] Laroche-R; Petat-E; Hajayandi-PC Human brucellosis in Burundi. Serologic survey in Rusizi Plain.Med-Trop-Mars. 1987 , 47(1): 35-8
[12] AItwegg-M; BohI-E . EvaIuation of a rapid, reliable, and inexpensive screening test for the serological diagnosis of human brucellosis. Zentralbl-BakterioI-MikrobioI-Hyg-A. 1985.