Determination of tetracycline hydrochloride in some pharmaceutical products using flow injection spectrophotometric technique

Shirwan Omer Baban, Rasul Jameel Ali
Chemistry Department, College of Education Science, Salahaddin University-Hawler, Iraqi-Kurdistan Region



A simple, rapid, and sensitive flow injection spectrophotometric system for
determination of tetracycline hydrochloride (TCH) in pharmaceutical
products. The method is based on diazotization of 2-aminopyridine and
subsequent coupling with tetracycline hydrochloride in alkaline medium. The
formed azo-dye showed maximum absorbance at 388 nm and the calibration
curve in the linear range of 25.0-150.0 µg/ml of tetracycline hydrochloride
with correlation coefficient 0.9988, while the detection limit was 10 µg/ml
and sample frequency 123 samples/hour. The accuracy and precision data for
determination of TCH by flow injection analysis method for triplicate injection
at three concentration levels(25, 80 and 150 µg/ml) the relative standard
deviation (RSD %) are 1.04, 0.247 and 1.75%, and relative error percent (E rel )
are -3.92, +1.06 and -1.9%. The proposed method was successfully applied to
the determination of tetracycline hydrochloride in pharmaceutical products.
The results obtained were in good agreement with those obtained by
reference method.

Key Words:
tetracycline hydrochloride, 2- aminopyridine, flow injection spectrophotometric


1. Abrams, A. C.; Lammon, C. B., and Pennington, S. S., ''Clinical Drug Therapy'', (8th ed.), Lipincott Williams & Wilkins, pp. 515-527, (2007).

2. Ali, S.; Abdulla, M. N. and Jasim, H. A. J. of Basrah Researches (Sciences), 36(5), pp.73-78, (2010).

3. Darken, M. A., Berenson, H., Shirk R. J. and Sjolander N. O.; Applied and Environmental Microbiology, 8(1), 46-51, (1960).

4. Mccormack, W. M.; Chowdhury, A. M.; Jahangir, N.; Ahmed, A. B. F. and Mosley, W. H. Bull. Org. mond. Sante, 38, pp.787-792, (1968)

5. Katzung, B. G., ''Basic & Clinical Pharmacology'', (10th ed.), McGraw- Hill Education (Asia), pp. 745-748, (2007).

6. Saikali Z. and Singh, G. Anti-Cancer Drugs, 14(10), pp.773–778, (2003).

7. Nester E. W.; Anderson D. G.; Roberts C. E.; Pearsall, N. N.; Nester M. T. and Hurly, D., ''Microbiology a Human Perspective'', (4th ed.), McGraw-Hill high Companies INC., , pp. 513, (2001).

8. Ahluwalia V. K. and Chopra, M., ''Medical Chemistry'', New Delhi, India, pp. 203-204, (2008).

9. Yongnian N.; Deng N.; and Kokot, S.; Anal. Methods, 2, 1302- 1309, (2010).

10. khodavirdilo B.; Samadi, N.; and Khodavirdilo, S., Asian J. of Biomedical and Pharmaceutical Sciences, 2(14), pp.65-71, (2012).

11. Othman N. S. and Al-Ashow, R. J. Raf. J. Sci., 23(2), 72-84, (2012).

12. Liawruangrath, S. S , Liawruangrath B. B. , Watanesk S. S. and Ruengsitagoon W. W. Anal. Sci. 22(1), pp.15-19. (2006).

13. Masawat, P.; Liawruangrath, S.; and Upalee S., Mj. Int. J. Sci. Tech., 2(1), pp.201-209, (2008).

14. Thanasarakhan, W.; Kruanetr, S., Deming, R. L.; Liawruangrath, B.; Wangkarna, S.; and Liawruangrath, S. Talanta, 84, , pp.1401- 1409,(2011).

15. Sokola, J.; and Matisova, E. J. of Chromatography A, 669(1-2), , pp.75-80, (1994).

16. Cheng, Y. F.; Phillips, D. J.; and Neue, U. Chromatographia, 44(3-4), pp.187-190, (1997).

17. Pena A.; Carmona, A.; Barbosa, A.; Lino, C., Silveira, I. and Castillo, B. J. of Pharmaceutical and Biomedical Analysis, 18, , pp.839-845, (1998).

18. Jochsberger, T.; Cutie, A.; and Mills, J.; J. Pharm. Sci., 68(8), , pp.1061-1063, (1979).

19. He-you, H. , Zhi-ke, H. and Yun'e, Z. , Wuhan University J. of Natural Sciences, 5(1), pp.93-97, (2000).

20. Chang, W. B.; Zhao, Y. B.; Xiang, Y. C. and Hu, L. Y. Fresenius J. of Analytical Chemistry, 344(3), pp.128-130, (1992).

21. Fahelelbom, K. M. S., American J. of Pharmacology and Toxicology, 3(3), pp.212-218, (2008).

22. Han, S. Q.; Liu, E. B.; and Li H. Chinese Chemical Letters 16(8), , 1067-1070, (2005).

23. Rufino J. L.; Fernandes, F. C. B.; Ruy M. S., Pezza, H. R.; and Pezza, L. Ecl. Quím. , 35(4), 139-146, (2010).

24. Townshenda, A.; Ruengsitagoon, W., Thongpoon, C. and Liawruangrath, S. Analytica Chimica Acta, 541, pp.105-111, (2005).

25. Zheng, X., Mei, Y. and Zhang, Z., Analytica Chimica Acta, 440, , pp.143-149, (2001).

26. Valcacel, M.; and decastro, M. D. L. '' Flow-Injection Principle and Applications'', (1st ed), Horwood limired English, p. 27, (1987)

27. Abdul, H., M. Sc. Thesis, University of Salahaddin-Erbil, , p. 56, (2007).

28. A. F. Jalal, M. Sc. Thesis, University of Salahaddin-Erbil, pp. 36-57, (2011).

29. British Pharmacopoeia, (4th ed.), Pharm. Press London, (2007),