Microbiological and mycotoxin risk assessment in ruminant feeding in Sulaimani governorate

Hozan Jalil Hamasalim, Karwan Muhamad Hama Khan & Sarwar Muhamad Sadq
Department of Animal Production, Faculty of Agricultural Sciences, University of sulaimani


Abstract

Background: Feed contamination and poisoning by microbial and fungal genera pose
obvious health threat to animals. Method: Vesa, ZAB, MIK, Deern, Zano and Zizar types
of ruminant feed used in Sulaimani Governorate were analyzed for their bacterial and
fungal quality. Identification of fungal isolates with determination of aflatoxin,
ochratoxin and T-2 toxin for all trademarks and local feed and the prime target of this
research would be background foreshadow for some danger of ruminant feedstuff in
Sulaimani governorate and taking these aspects in consideration to assess
microbiological quality and mycotoxins in ruminant feed. Result: The genera of bacteria
and fungi isolated and their occurrence were Salmonella sp., total plate count and total
fungal count. Based on standard method identification of fungal species in brands
ruminant feed and local ruminant feed, the all commonly isolated genera were
Aspergillus spp., Penicillium spp and Fusarium spp. Finally, detected concentration of
each aflatoxin, ochratoxin and T-2 toxin in brands ruminant feed and local ruminant
feed. Most types of ruminant feed under study were lower than the maximal standard
limits of aflatoxin while all types of brands ruminant feed and local ruminant feed under
study were lower than the maximal tolerance limits of ochratoxin and T-2 toxin.

Key Words
Ruminant Feed, Microbiological Quality, Identification of Fungal and Mycotoxin.

References

[1] Abedullah, N. Mahmood, M. K. and Kouser, S., “The role of agricultural credit in the growth of livestock sector: A case study of Faisalabad” Pakistan Vet. J., 29(2): 81-84, (2009).

[2] Addinsoft, “LSTAT 2015.1” EULA. Read EULA. EULA.XLSTAT Version 2015.1.03.15828. Copyright addinsoft : 2-14, (2015).

[3] Akande, K. E., Abubakar, M. M., Adegbola, T. A. and Bogoro, S. E., “Nutritional and health implications of mycotoxin in animal feed” Pakistan J. Nutr., 5(5): 398-403, (2006).

[4] Basu, M., Seggerson, S., Henshaw, J., Jiang, J., Rocio, A. C., Clare, L., Boyle, P., Miller, A., Pugia, M. and Basu, S., “Nano-biosensor development for bacterial detection during human kidney infection: Use of glycoconjugate-specific antibodybound gold nano wire arrays (GNWA)”  Glycoconjugate Journal. 21 (8-9):487- 496, (2004).

[5] Bryden, W. L., “Mycotoxins and Animal Production: Insidious Problems Associated with Contaminated Feedstuffs” In Proceedings of the International Symposium on Recent Advances in Animal Nutrition, Kuala Lumpur, Malaysia, (2004).

[6] Chahal, U. S., Niranjan, P. S. Sanjay, K.,  “Handbook of General Animal Nutrition” International book distributing co., 81-8189: 176-7, (2008).

[7] D’Mello, J. P. F., Macdonald, A. M. C., “Mycotoxins” Anim. Feed Sci. Technol., 69: 155–166, (1997).

[8] David, M. W., Wellington, M. and Zeljko, J., “Biology and ecology of mycotoxigenic Aspergillus species as related to economic and health concerns, In: Mycotoxins and food safety”  DeVries, W., Truksess, W. and Jackson, L.S.  Advances in experimental medicine and biology. Kluwer Academic/Plenum Publishers.USA., 504 :3-17, (2002).

[9] Drasar, B. S. and Barrow, P. A., “Intestinal Microbiology” In: (eds), Amer. Soc. Microbiol. Press, Washington, DC pp.:19-40, (1985).

[10] Duarte, S. C., Lino, C. M., Pena, A. and Ochratoxin, A., “In feed of food-producing animals: An undesirable mycotoxin with health and performance effects” Vet. Microbiol., 154: 1– 3, (2011).

[11] European Union, “control of salmonella and other specified foodborne zoonotic agents” Regulation (EC) No. 2160/2003 of the European Parliament and of the Council of 17 November 2003  . Off. J. Eur. Union, L 325 of 12.12.2003, 1-15, (2003).

[12] FAO, “Quality assurance for microbiology in feed analysis laboratories”  by R.A. Cowie. Edited by Harinder P.S. Makkar. FAO, Animal Production and Health Manual No. 16. Rome, (2013).

[13] Fraizier, W. C. and Westhoff, D. C., “Food Microbiology” 3rd edition. Tata McGraw Hill publishing company Limited, New Delhi, (1978).

[14] Frisvad, J. C. and Thrane, U., “ Mycotoxin production by common filamentous fungi” In: Samson R. A., Hoekstra, E. S., Frisvad, J. C. and Filtenborg, O. (Eds): Introduction to Food- and Airborne Fungi., Central bureau voor Schimme cultures, Utrecht, 321-330, (2002).

[15] Hiba, S. A., “Estimation of aflatoxin levels for some raw milk types and cheeses (Local & imported) in Mosul city” (Masters thesis) Mosul.Iraq. 115, (2007).

[16] Hoszowski, A. W. D., “Salmonella prevalence and resistance to antibiotics in Poland”  Med Wet., 6: 660-663, (2005).

[17] IARC (International Agency for Research on Cancer) , “Some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins—Fumonisin B1” IARC Monogr. Eval. Carcinog. Risks Hum., 56: 245–395,( 1993).

[18] Jensen, A. N., Lodal, J. and Baggesen, D. L., “High diversity of Salmonella serotypes found in an experiment with outdoor pigs” NJAS-Wageningen J., Life Sci., 52: 109–117,( 2004).

[19] Juven, B. J., Cox, N. A., Bailey, J. S., Thomaon, J. E., Charles, O.W. and Shutze, J. V., “Survival of Salmonella in dry food and feed” J. Food Prot., 47(6): 445–448, (1984).

[20] Kwiatek, K., Przeniosło, M., Weiner, A. and Wojdat, E., “Jakość środków żywienia zwierząt a zapewnienie bezpieczeństwa żywności pochodzenia zwierzęcego” Higiena. 4: 5-9, (2002).

[21] Laven, R. A., Ashmore, A. and Stewart, C. S., “Escherichia coli in the rumen and colon of slaughter cattle, with particular reference to E. coli O157” Vet., J., 165:78-83,( 2003).

[22] Lee-Jiuan, C. and Li-Mien, T., “High occurrence of mycotoxins in Asian feedstuffs” World poultry. 10(4):13-16,( 2006).

[23] Min, B. R., Pinchak, W. E., Anderson, R. C. and Callaway, T. R., “Effect of tannins on the in vitro growth of Escherichia coli O157:H7 and in vivo growth of generic Escherichia coli excreted from steers” J., Food Prot., 70:543-550, (2007).

[24] Misra, J. K., Gergon, E. B. and Mew, T. W., “Storage fungi of rice in the Phillipines” Mycopathologia. 13 (1): 13-24,( 1995).

[25] Ogbulie, J. N., “Microbial ecology of tropical aquatic system” Ph. D thesis. University of Port-Harcourt Nigeria,( 1995).

[26] OSP (Opinion of the Scientific Panel). “Contaminants in Food Chain on a request from the Commission related to ochratoxin A (OTA) as undesirable substance in animal feed” EFSA J. 2004, 101, 1–36,( 2004).

[27] Pacin, A. M., González, H. H. L., Etcheverry, M., Resnik, S. L., Vivas, L. and Espin, S. “Fungi associated with food and feed commodities from Ecuador” J. 156: 87-92,( 2003).

[28] Pitt, J. I., “Penicillium viridicatum, Penicillium verrucosum and production of ochratoxin A” Appl Environm Microbiol., 53: 266-269, (1987).

[29] Pitt, J. I., Hocking, A. D., Kanjana, B., Miseamble, B. F., Wheeler, K. A. and Tanboon, P., “The normal mycoflora of commodities form Thailand Rice, Beans and other commodities” International Journal of Environmental Health Research. 4: 102 – 108,(1994).

[30] Samson, R. A. “Polyphasic taxonomy of Penicillium subgenus Penicillium. A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins” Stud Mycol., 49: 1-173,( 2004).

[31] Wasyl, D., “Występowanie pałeczek Salmonella u zwierząt, w środkach żywienia zwierząt i żywności w pierwszej połowie” Conference Proceedings, National Veterinary Research Institute, Puławy,( 2003).

[32] Winfield, M. D. and Groisman, E. A. “Role of nonhost environments in the lifestyles of Salmonella and Escherichia coli” Appl. Environ. Microbiol. 69: 3687-3694,( 2003).

[33] Wogan, G. N., “Chemical Nature and Biological Effects of the Aflatoxins” Bacteriol. Rev., 30: 460–470, (1966).

[34] Wojdat, E., Krzysztof,  K. and Magdalena, K. 2005. Microbiological Quality of Animal Feedingstuffs in Poland. Bull Vet Inst Pulawy 49: 315-318.

[35] World Health Organization (WHO). “Report of the WHO Consultation on control of Salmonella infections in animals: prevention of food borne Salmonella infections in humans” Jena, Germany, 21-26 November. WHO/CDS/VPH/93.129. WHO, Geneva, (1993).

[36] Yegani, M., Smith, T. K. and Lesson, S. B., “Effects of feeding grains naturally contaminated with Fusarium mycotoxins on performance and metabolism of broiler breeders” Poult. Sci., 85: 1541- 1549,( 2006).

[37] Zhao, S., Blickenstaff, K. and Glenn, A. “Lactam resistance in Salmonella strains isolated from retail meats in the United States by the National Antimicrobial Resistance Monitoring System between 2002 and 2006” Appl Environ Microbiol., 75: 7624–7630,( 2009).