Sequence Verifications and Promoter Analysis of The Prolactin Gene
Keywords:Prolactin, Hyperprolactinemia, Infertility, Gene expression, Promoter analysis
AbstractProlactin or luteotropic hormone (PRL) is a protein hormone. Hyperprolactinemia is one of the most common endocrine disorders. We initiate to investigate if the genetic factor was behind some of the infertile cases suffering from hyperprolactinemia in slemani city. For this study, 36 samples from infertile women were taken and the genomic DNA was isolated. We desgined specific polynucleotide primers to amplify a portion of the PRL gene using PCR technique. The PCR products were subjected for DNA sequencing together with PCR products of 15 samples from healthy individuals. Silent mutations were found between individuals, but non of them correlated to the PRL gene variations between healthy and hyperprolactinemic cases. We perform promoter analysis of the PRL gene using Bioinformatics approcach to identify/determine specific transcription regulator(s) of the PRL gene.
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