Sequence Verifications and Promoter Analysis of The Prolactin Gene
DOI:
https://doi.org/10.17656/jzs.10234Keywords:
Prolactin, Hyperprolactinemia, Infertility, Gene expression, Promoter analysisAbstract
Prolactin or luteotropic hormone (PRL) is a protein hormone. Hyperprolactinemia is one of the most common endocrine disorders. We initiate to investigate if the genetic factor was behind some of the infertile cases suffering from hyperprolactinemia in slemani city. For this study, 36 samples from infertile women were taken and the genomic DNA was isolated. We desgined specific polynucleotide primers to amplify a portion of the PRL gene using PCR technique. The PCR products were subjected for DNA sequencing together with PCR products of 15 samples from healthy individuals. Silent mutations were found between individuals, but non of them correlated to the PRL gene variations between healthy and hyperprolactinemic cases. We perform promoter analysis of the PRL gene using Bioinformatics approcach to identify/determine specific transcription regulator(s) of the PRL gene.References
-Yen S. S. C, Jaffe R. B, and Barbieri R. L, “Reproductive endocrinology” 4th edition, W.B. Saunders Co, Philadelphia, pp. 257-283, (1999).
-Woodman D. D, “Laboratory animal endocrinology” Chichester, UK, (1997).
-Bole-Feysot, C., Goffin, V., Edery, M., Binart, N. and Kelly, P. A, “Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice” Endocr. Rev. 19, pp.225-268, (1998).
-Noh G. E, Lim H. K. and Kim J. M, “Characterization of genes encoding prolactin and prolactin receptors in starry flounder Platichthys stellatus and their expression upon acclimation to freshwater” Fish Physiol. Biochem. pp. 608-737, (2012).
-Berwaer M, Martial J. A, and Davis J. R, “Characterization of an upstream promoter directing extrapituitary expression of the human prolactin gene” Mol. Endocrinol. 8, pp. 635-642, (1994).
-Binart N, Bachelot A,and Bouilly J, “Impact of prolactin receptor isoforms on reproduction” Trends Endocrinol. Metab. 21(6), pp. 362-368, (2010).
-Mah P. M and Webster J, “Hyperprolactinemia: etiology, diagnosis, and management” Semin Reprod Med. 20, pp. 365-374, (2002).
-Luciano A. A, “Clinical presentation of hyperprolactinemia” J. Reprod. Med., 1999, 44, 1085-1090.
-Biller B. M, “Diagnostic evaluation of hyperprolactinemia” J. Reprod. Med. 44, pp. 1095-1099, (1999).
-Dyar A. H, “detection of Brucellosis causing bacteria (Brucella SPP.) by polymerase chain reaction (PCR)” High Diploma thesis, Sulaimani university, 2009.
-Miller S. A., Dykes D. D., and Polesky H. F, “a simple salting out procedure for extracting DNA from human nucleated cells” Nucl. Acids Rev. 16, pp.1215, (1988).
-Matys V, Fricke E, Geffers R, Gössling E, Haubrock M, Hehl R, et al, “TRANSFAC: transcriptional regulation, from patterns to profiles” Nucleic Acids Res. 31, pp. 374-378, (2003).
-Chekmenev D. S, Haid C, and Kel A. E, “P-Match :transcription factor binding site search by combining patterns and weight matrices” Nucleic Acids Res. 33, pp. W432-W437, (2005).
-Li B, Liu L, Fu X, Zhou W. Q, Zou D. T, Zhao X. Y et al, “A novel mutation of Estrogen receptor gene detected in girls with precocious puberty” Acta. Genetica. Sinica. 32, pp. 1011-1017, (2005).
-Vikas A, Thayanithy V, Ramanathan K, Thavamani J. P, and Sinnakaruppan M, “Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis)” J. Biosci. 26, pp. 315-324, (2001).
-Janeth S. P, José L. Caldas W, Ronaldo de C. A. and Alexandre W. S. “Molecular cloning and sequence analysis of growth hormone cDNA of Neotropical freshwater fish Pacu (Piaractus mesopotamicus)” Genetics and Molecular Biology. 31, pp. 381-384, (2008).
-Krumlauf R, “Hox genes in vertebrate development” Cell. 78, pp. 191-201, (1994).
-Aubin J, Lemieux M, Tremblay M, Be´rard J, and Jeannotte L, “Early postnatal lethality in Hoxa-5 mutant mice is attributable to respiratory tract defects” Dev. Biol. 192, pp. 432-445, (1997).
-Christian L, Michel T, Daniel B, Josee A, and Lucie J, “Multiple cis-acting regulatory regions are required for restricted spatio-temporal Hoxa5 gene expression” Dev. Dyn. 214, pp. 127-140, (1999).
-Shawn P. M, James G, Ward F. O, Robert A. L, and Elwood L, “Differential expression of the homeobox gene Hox-1.3 in F9 embryonal carcinoma cells” Proc. Natl. Acad. Sci. 85, pp. 5587-5591, (1988).
-El-Hodiri H, Bhatia-Dey N, Kenyon K, Ault K, Dirksen M, and Jamrich M, “Fox (forkhead) genes are involved in the dorso-ventral patterning of the Xenopus mesoderm” Int. J. Dev. Biol. 45(1), pp. 265-271, (2001).
-Gómez-Ferrería M. A. and Rey-Campos J, “Functional domains of FOXJ2” J. Mol. Biol. 329(4), pp. 631-644, (2003).
-Bülow M. H, Aebersold R, Pankratz M. J, Jünger M. A, “The Drosophila FoxA ortholog Fork head regulates growth and gene expression downstream of Target of rapamycin, PLoS One. 5(12):e15171, (2010).
-Norquay L. D, Yang X, Jin Y, Detillieux K. A, Cattini P. A, “Hepatocyte nuclear factor-3alpha binding at P sequences of the human growth hormone locus is associated with pituitary repressor function” Mol. Endocrinol. 20(3), pp. 598-607, (2006).
-Kaestner K. H., Knochel W., and Martinez D. E, “unified nomenclature for the winged helix/forkhead transcription factors” Genes Dev. 15, pp. 142-146, (2000).
-Jackson B C, Carpenter C, Nebert D. W, Vasiliou V, “Update of human and mouse forkhead box (FOX) gene families” Hum. Genomics. 4(5), pp. 345-352, (2010).
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