Characterization of the flavonolignans in Silybummarianum L. Grown Naturally in Iraqi-Kurdistan Region


  • Mohammed Nawzad Sabir School of Pharmacy, University of Sulaimani, Kurdistan Region, Iraq. Author
  • Ali Abdulrahman Askari School of Pharmacy, University of Sulaimani, Kurdistan Region, Iraq. Author



Flavonolignans, Silybins, Chromatography


(Abstracted from MSc. Thesis entitles "Pharmacognostic and Pharmacologic study of Silybum marianum grown naturally in Iraqi-Kurdistan Region")  The antihepatotoxic principles found in the indigenous plant Silybum marianum L. (Milk thistle) was obtained using a multi-extraction/ separation and isolation procedures. Starting with identification of the plant and collection of the seeds. A pretreatment (defatting) step with light petroleum ether preceded the extraction with organic solvent (ethanol) in a soxhlet extractor. The alcoholic extract after drying was partitioned with n-hexane, Chloroform, Ethyl acetate, and n-butanol successively. Each fraction was separated by flash column chromatography. The sub-fractions after column chromatography were tested with thin layer chromatography (TLC) for the presence of the flavonolignans. Fractions showed spots with the same Rf-values were combined and furthermore isolated with high performance liquid chromatography (HPLC). Components were then purified and identified using UV, IR-spectroscopy. The results were compared with a standard sample of silybin (obtained from Sigma-Aldrich Co.). Results confirmed that the plant Silybum marianum L. which grows indigenous in Kurdistan Region/ Iraq, reserves the same active components (flavonolignans) present in the plant that grows elsewhere.


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How to Cite

Characterization of the flavonolignans in Silybummarianum L. Grown Naturally in Iraqi-Kurdistan Region. (2013). Journal of Zankoy Sulaimani - Part A, 15(4), 1-12.