Identification of Animal Species in Meat Broth by Simplex and Multiplex PCR

Authors

  • Peshnyar Muhammad Atta Rashid Molecular Diagnostic Laboratory, Sulaimani Veterinary Directorate, Kurdistan Region, Iraq. Author
  • Muhamad Omer Babashekh Molecular Diagnostic Laboratory, Sulaimani Veterinary Directorate, Kurdistan Region, Iraq. Author
  • Ari Salahadin Marouf Molecular Diagnostic Laboratory, Sulaimani Veterinary Directorate, Kurdistan Region, Iraq. Author
  • Kamaran Mohamad Amin Molecular Diagnostic Laboratory, Sulaimani Veterinary Directorate, Kurdistan Region, Iraq. Author

DOI:

https://doi.org/10.17656/jzs.10288

Keywords:

Meat broth sample, Species specific primer, Veterinary forensic medicine, Meat identification

Abstract

In some countries, some restaurants may use fraudulent substitution in meat product that has less commercial value or undesirable. Effective laboratory tests must be available for prevention of this phenomenon. In this study meat broth sample was successfully used to identify seven animal species in cooked meals by simplex and multiplex PCR using species specific primers for conserved region of mitochondrial DNA (mtDNA). The results showed effective amplification of mtDNA without cross reaction between primers. Whereas existing methods require mincing and homogenization of direct meat samples, this meat broth sampling method can be performed directly and is thus easier to perform. In conclusion, meat broth sample could be used instead of direct meat tissues for species identification of cooked meat in restaurants to control adulteration substitutions of undesirable meat and protection of the customer rights.

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Published

2014-02-24

How to Cite

Identification of Animal Species in Meat Broth by Simplex and Multiplex PCR. (2014). Journal of Zankoy Sulaimani - Part A, 16(1), 97-102. https://doi.org/10.17656/jzs.10288