Indirect spectrophotometric determination of folic acid based on the oxidation reaction and studying some of the thermodynamic parameters


  • N. S. Othman College of Science, University of Mosul, Kurdistan Region, Iraq. Author
  • Sh. H. Hasan College of Agriculture, University of Salahaddin, Erbil, Kurdistan Region, Iraq. Author
  • K. M. Surchi College of Science, University of Salahaddin, Erbil, Kurdistan Region, Iraq. Author



Spectrophotometry, Folic acid, 2,2'-Bipyridyl/FeCl3 Thermodynamic, Parameters


A simple and sensitive indirect spectrophotometric method was developed for the determination of folic acid in pure form and in its pharmaceutical formulations. The method was based on the oxidation of folic acid by iron (III) in acidic medium, then reaction of liberated iron (II) with 2, 2'-bipyridyl to produce a red complex which is water-soluble, stable, and has a maximum absorption at 520 nm against the reagent blank with a molar absorptivity of 2.8338 x 105 L Beer’s law is valid over the concentration range of 0.5-18.0 µg/ml folic acid. The values of some thermodynamic parameters [free energy change (∆G), enthalpy change (∆H), and entropy change (∆S)] were estimated from the values of stability constants (K) at different temperatures .It was found  that  colored product formation was spontaneous, exothermic and accompanied by an increase of random. The proposed method has been applied successfully to determine folic acid in different pharmaceutical preparations.


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How to Cite

Indirect spectrophotometric determination of folic acid based on the oxidation reaction and studying some of the thermodynamic parameters. (2014). Journal of Zankoy Sulaimani - Part A, 17(1), 61-70.