Zones of maximum discontinuity in the western house mouse (M. m. domesticus) genetic landscape: a computational geometry method.


  • Fatah Zarei Graduate Student of Systematic Zoology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran. Author
  • Ridha H. Hussein Zoology Assistant Professor, Department of Biology, School of Science, University of Sulaimani, Kurdistan Region, Iraq. Author



BARRIER analysis, Genetic discontinuity, Monmonier’s algorithm, Western house mouse


To represent the zones of maximum genetic discontinuity in a network of geographically located populations of western house mouse from the previously published mitochondrial DNA D-loop sequence datasets which we acquired from the NCBI GenBank database, we applied a computational geometry approach using the Monmonier’s maximum difference algorithm implemented in the BARRIER 2.2 software. We defined ten zones where genetic change among populations is locally increased, three of them occur within the Middle East, while seven occur in Europe. Analysis of molecular variance among post-hoc defined regions using the pattern generated by the BARRIER analysis showed that a significant portion of the genetic diversity was because of among groups component.


A. Banaszek, J. Ziomek, K. A. Jadwiszczak, E. Kaczyńska, and P. Mirski, "Identification of the barrier to gene flow between phylogeographic lineages of the common hamster Cricetus cricetus" Acta. Theriol. 57(3), pp. 195-204, (2012). DOI:

E. Mayr, "Populations, species, and evolution: an abridgment of animal species and evolution", Harvard University Press, pp. 297-336, (1970).

R. Sage, "Wild mice" The mouse in biomedical research 1, pp. 39-90, (1981).

E. Schwarz and H. K. Schwarz, "The wild and commensal stocks of the house mouse, Mus musculus Linnaeus" J. Mammal. 24(1), pp. 59-72, (1943). DOI:

T. Cucchi, J. D. Vigne, and J. C. Auffray, "First occurrence of the house mouse (Mus musculus domesticus Schwarz & Schwarz, 1943) in the Western Mediterranean: a zooarchaeological revision of subfossil occurrences" Biol. J. Linn. Soc. 84(3), pp. 429-445, (2005). DOI:

T. Cucchi, "Uluburun shipwreck stowaway house mouse: molar shape analysis and indirect clues about the vessel's last journey" J. Archaeol. Sci. 35(11), pp. 2953-2959, (2008). DOI:

J.-C. Auffray, E. Tchernov, F. Bonhomme, G. Heth, S. Simson, and E. Nevo, "Presence and ecological distribution of Mus spretoides and Mus musculus domesticus in Israel Circum-Mediterranean vicariance in the genus Mus" Zeitschrift für Säugetierkunde 55(1), pp. 1-10, (1990).

H. Rajabi-Maham, A. Orth, and F. Bonhomme, "Phylogeography and postglacial expansion of Mus musculus domesticus inferred from mitochondrial DNA coalescent, from Iran to Europe" Mol. Ecol. 17(2), pp. 627-641, (2008). DOI:

S. I. Gabriel, M. D. L. Mathias, and J. B. Searle, "Genetic structure of house mouse (Mus musculus Linnaeus 1758) populations in the Atlantic archipelago of the Azores: colonization and dispersal" Biol. J. Linn. Soc. 108(4), pp. 929-940, (2013). DOI:

İ. Gündüz, R. V. Rambau, C. Tez, and J. B. Searle, "Mitochondrial DNA variation in the western house mouse (Mus musculus domesticus) close to its site of origin: studies in Turkey" Biol. J. Linn. Soc. 84(3), pp. 473-485, (2005). DOI:

F. Manni, E. Guerard, and E. Heyer, "Geographic patterns of (genetic, morphologic, linguistic) variation: how barriers can be detected by using Monmonier's algorithm" Hum. Biol. 76(2), pp. 173-190, (2004). DOI:

M. S. Monmonier, "Maximum‐Difference Barriers: An Alternative Numerical Regionalization Method" Geogr. Anal. 5(3), pp. 245-261, (1973). DOI:

K. Katoh, K.-i. Kuma, H. Toh, and T. Miyata, "MAFFT version 5: improvement in accuracy of multiple sequence alignment" Nucleic. Acids. Res. 33(2), pp. 511-518, (2005). DOI:

D. Posada, "jModelTest: phylogenetic model averaging" Mol. Biol. Evol. 25(7), pp. 1253-1256, (2008). DOI:

L. Excoffier and H. E. Lischer, "Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows" Mol. Ecol. Resour. 10(3), pp. 564-567, (2010). DOI:

K. E. Brassel and D. Reif, "A procedure to generate Thiessen polygons" Geogr. Anal. 11(3), pp. 289-303, (1979). DOI:

G. Voronoï, "Nouvelles applications des paramètres continus à la théorie des formes quadratiques. Deuxième mémoire. Recherches sur les parallélloèdres primitifs" J. Reine. Angew. Math. 1908(134), pp. 198-287, (1908). DOI:

L. Excoffier, P. E. Smouse, and J. M. Quattro, "Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data" Genetics 131(2), pp. 479-491, (1992). DOI:

M. Hasegawa, H. Kishino, and T.-a. Yano, "Dating of the human-ape splitting by a molecular clock of mitochondrial DNA" J. Mol. Evol. 22(2), pp. 160-174, (1985). DOI:

Z. Yang, "Maximum likelihood phylogenetic estimation from DNA sequences with variable rates over sites: approximate methods" J. Mol. Evol. 39(3), pp. 306-314, (1994). DOI:

J. Britton-Davidian "Genic differentiation in M. m. domesticus populations from Europe, the Middle East and North Africa: geographic patterns and colonization events" Biol. J. Linn. Soc. 41(1‐3), pp. 27-45, (1990). DOI:

R. D. Sage, E. Prager, H. Tichy, and A. C. Wilson, "Mitochondrial DNA variation in house mice, Mus domesticus (Rutty)" Biol. J. Linn. Soc. 41(1‐3), pp. 105-123, (1990). DOI:

F. Bonhomme, A. Orth, T. Cucchi, H. Rajabi-Maham, J. Catalan, P. Boursot, J.-C. Auffray, and J. Britton-Davidian, "Genetic differentiation of the house mouse around the Mediterranean basin: matrilineal footprints of early and late colonization" P. Roy. Soc. B- Biol. Sci. 278(1708), pp. 1034-1043, (2011). DOI:

E. Solano, P. Franchini, P. Colangelo, E. Capanna, and R. Castiglia, "Multiple origins of the western European house mouse in the Aeolian Archipelago: clues from mtDNA and chromosomes" Biol. Invasions 15(4), pp. 729-739, (2013). DOI:

H. Suzuki, M. Nunome, G. Kinoshita, K. P. Aplin, P. Vogel, A. P. Kryukov, M.-L. Jin, S.-H. Han, I. Maryanto, and K. Tsuchiya, "Evolutionary and dispersal history of Eurasian house mice Mus musculus clarified by more extensive geographic sampling of mitochondrial DNA" Heredity 111(5), pp. 375-390, (2013). DOI:

T. Cucchi, Z. E. Kovács, R. Berthon, A. Orth, F. Bonhomme, A. Evin, R. Siahsarvie, J. Darvish, V. Bakhshaliyev, and C. Marro, "On the trail of Neolithic mice and men towards Transcaucasia: zooarchaeological clues from Nakhchivan (Azerbaijan)" Biol. J. Linn. Soc. 108(4), pp. 917-928, (2013). DOI:

H. Rajabi‐Maham, A. Orth, R. Siahsarvie, P. Boursot, J. Darvish, and F. Bonhomme, "The south‐eastern house mouse Mus musculus castaneus (Rodentia: Muridae) is a polytypic subspecies" Biol. J. Linn. Soc. 107(2), pp. 295-306, (2012). DOI:

M. Macholán, M. M. Vyskočilová, V. Bejček, and K. Šťastný, "Mitochondrial DNA sequence variation and evolution of Old World house mice (Mus musculus)" Folia. Zool. 61(3-4), pp. 284-307, (2012). DOI:

A. Orth, J. Auffray, and F. Bonhomme, "Two deeply divergent mitochondrial clades in the wild mouse Mus macedonicus reveal multiple glacial refuges south of Caucasus" Heredity 89(5), pp. 353-357, (2002). DOI:

R. Castiglia, F. Annesi, and E. Capanna, "Geographical pattern of genetic variation in the Robertsonian system of Mus musculus domesticus in central Italy" Biol. J. Linn. Soc. 84(3), pp. 395-405, (2005). DOI:

M. Linnenbrink, J. Wang, E. A. Hardouin, S. Künzel, D. Metzler, and J. F. Baines, "The role of biogeography in shaping diversity of the intestinal microbiota in house mice" Mol. Ecol. 22(7), pp. 1904-1916, (2013). DOI:

E. A. Hardouin, J.-L. Chapuis, M. I. Stevens, J. B. van Vuuren, P. Quillfeldt, R. J. Scavetta, M. Teschke, and D. Tautz, "House mouse colonization patterns on the sub-Antarctic Kerguelen Archipelago suggest singular primary invasions and resilience against re-invasion" BMC Evol. Biol. 10(1), pp. 325, (2010). DOI:

A. Geraldes, P. Basset, B. Gibson, K. L. Smith, B. Harr, H. T. YU, N. Bulatova, Y. Ziv, and M. W. Nachman, "Inferring the history of speciation in house mice from autosomal, X‐linked, Y‐linked and mitochondrial genes" Mol. Ecol. 17(24), pp. 5349-5363, (2008). DOI:

D. Förster, I. Gündüz, A. Nunes, S. Gabriel, M. Ramalhinho, M. Mathias, J. Britton-Davidian, and J. Searle, "Molecular insights into the colonization and chromosomal diversification of Madeiran house mice" Mol. Ecol. 18(21), pp. 4477-4494, (2009). DOI:

E. P. Jones, F. Jóhannesdóttir, I. Gündüz, M. B. Richards, and J. Searle, "The expansion of the house mouse into north‐western Europe" J. Zool. 283(4), pp. 257-268, (2011). DOI:

J. B. Searle, C. S. Jones, İ. Gündüz, M. Scascitelli, E. P. Jones, J. S. Herman, R. V. Rambau, L. R. Noble, R. Berry, and M. D. Giménez, "Of mice and (Viking?) men: phylogeography of British and Irish house mice" P. Roy. Soc. B- Biol. Sci. 276(1655), pp. 201-207, (2009). DOI:



How to Cite

Zones of maximum discontinuity in the western house mouse (M. m. domesticus) genetic landscape: a computational geometry method. (2016). Journal of Zankoy Sulaimani - Part A, 18(2), 349-364.