The Estimate of Cytokines and Fibroblast Growth Factors in Patients with Breast Cancer

Authors

  • Shvan Burhan Rafiq Department of Clinical Biochemistry, College of Pharmacy, University of Sulaimani, Kurdistan Region, Iraq. Author
  • Heshu Sulaiman Rahman Department of Physiology, College of Medicine, University of Sulaimani, Kurdistan Region, Iraq. Author
  • Kawa Amin Department of Microbiology/Immunology, College of Medicine, University of Sulaimani, Kurdistan Region, Iraq. & Department of Medical Science, Respiratory Medicine and Allergology, Uppsala University and University Hospital, Uppsala, Sweden. Author

DOI:

https://doi.org/10.17656/jzs.10803

Keywords:

Breast cancer, Cytokines, Fibroblast, Immune system, Inflammation, ELISA

Abstract

Breast cancer is the most common cancer in women worldwide; it is a disease characterized by the growth of malignant cells within the mammary glands. It strikes males and females; there are diverse sorts of breast cancer. Cytokines and fibroblast growth factors that play a critical role in the development and progression of breast cancer. The aim of the present study is to assess the role and the level of certain cytokines, and fibroblast growth factors in the serum of patients with breast cancer. This is case-control study, blood samples were collected randomly from 60 newly diagnosed female patients, and 60 healthy female subject controls of all age groups. Venous blood samples were collected and analyzed for cytokines (Interleukin-19 and Interleukin-23) and fibroblast growth factors (FGF-18 and FGF-23) by using ELISA. There was a highly significant difference (p <0.001) detected in serum IL-19, IL-23, and FGF-18 levels of patients compared to healthy controls except for FGF-23, which was also recorded higher value in patients but not significantly compared to controls. There was a significant positive correlation (p <0.001) between all these markers. The present study reveals significantly higher concentrations of the markers detected in patients’ blood serum compared to healthy individuals. The detection of the cytokines’ levels and their relations with breast cancer will affect the diagnosis, knowledge about the disease immunology and thereafter effects on the treatments of patients with breast cancer depending on our findings.

References

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. "Global cancer statistics 2012". CA Cancer J Clin. Vol. 65, No. 2, pp. 87-108. 2015). DOI: https://doi.org/10.3322/caac.21262

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. "Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries". CA Cancer J. Clin. Vol. 68, No. 6, pp. 394-424. (2018). DOI: https://doi.org/10.3322/caac.21492

Moodley J, Cairncross L, Naiker T, Constant D. "From symptom discovery to treatment - women's pathways to breast cancer care: a cross-sectional study". BMC Cancer. Vol. 18, No. 1, pp. 312. (2018). DOI: https://doi.org/10.1186/s12885-018-4219-7

Yager JD, Davidson NE. "Estrogen carcinogenesis in breast cancer". N. Engl J. Med. Vol. 354, No. 3, pp. 270-82. (2006). DOI: https://doi.org/10.1056/NEJMra050776

Dmitrieva OS, Shilovskiy IP, Khaitov MR, Grivennikov SI. "Interleukins 1 and 6 as Main Mediators of Inflammation and Cancer". Biochemistry (Mosc). Vol. 81, No. 2, pp. 80-90. (2016). DOI: https://doi.org/10.1134/S0006297916020024

Paduch R, Walter-Croneck A, Zdzisińska B, Szuster-Ciesielska A, "Kandefer-Szerszeń M. Role of reactive oxygen species (ROS), metalloproteinase-2 (MMP-2) and interleukin-6 (IL-6) in direct interactions between tumour cell spheroids and endothelial cell monolayer". Cell Biol Int. Vol. 29, No. 7, pp. 497-505. (2005). DOI: https://doi.org/10.1016/j.cellbi.2005.01.007

Menon R, Ismail L, Ismail D, Merialdi M, Lombardi SJ, Fortunato SJ. "Human fetal membrane expression of IL-19 and IL-20 and its differential effect on inflammatory cytokine production". J. Matern. Fetal. Neona. Vol. 19, No. 4, pp. 209-214. (2006). DOI: https://doi.org/10.1080/14767050500440986

Tang C, Chen S, Qian H, Huang W. "Interleukin-23: as a drug target for autoimmune inflammatory diseases". Immunology. Vol. 135, No. 2, pp. 112-124. (2012). DOI: https://doi.org/10.1111/j.1365-2567.2011.03522.x

Sheng S, Zhang J, Ai J, Hao X, Luan R. "Aberrant expression of IL-23/IL-23R in patients with breast cancer and its clinical significance". Molecular medicine reports. Vol. 17, No. 3, pp. 4639-44. (2018). DOI: https://doi.org/10.3892/mmr.2018.8427

Diez del Corral R, Breitkreuz DN, Storey KG. "Onset of neuronal differentiation is regulated by paraxial mesoderm and requires attenuation of FGF signalling". Development. Vol. 129, No. 7, pp. 1681-1691. (2002). DOI: https://doi.org/10.1242/dev.129.7.1681

Majidinia M, Sadeghpour A, Yousefi B. "The roles of signaling pathways in bone repair and regeneration". J. Cell Physiol. Vol. 233, No. 4, pp. 2937-2948. (2018). DOI: https://doi.org/10.1002/jcp.26042

Yu Z, Lou L, Zhao Y. "Fibroblast growth factor 18 promotes the growth, migration and invasion of MDA‑MB‑231 cells". Oncology reports. Vol. 40, No. 2, pp. 704-714. (2018).

Ohbayashi N, Shibayama M, Kurotaki Y, Imanishi M, Fujimori T, Itoh N, et al. "FGF18 is required for normal cell proliferation and differentiation during osteogenesis and chondrogenesis". Genes Dev. Vol. 16, No. 7, pp. 870-879. (2002). DOI: https://doi.org/10.1101/gad.965702

Sonvilla G, Allerstorfer S, Stättner S, Karner J, Klimpfinger M, Fischer H, et al. "FGF18 in colorectal tumour cells: autocrine and paracrine effects". Carcinogenesis. Vol. 29, No. 1, pp. 15-24. (2008). DOI: https://doi.org/10.1093/carcin/bgm202

Wei W, Mok SC, Oliva E, Kim S-h, Mohapatra G, Birrer MJ. "FGF18 as a prognostic and therapeutic biomarker in ovarian cancer". J. Clin. Invest. Vol. 123, No. 10, pp. 4435-4448. (2013). DOI: https://doi.org/10.1172/JCI70625

Shimada T, Mizutani S, Muto T, Yoneya T, Hino R, Takeda S, et al. "Cloning and characterization of FGF23 as a causative factor of tumor-induced osteomalacia". Proc. Natl. Acad. Sci. USA. Vol. 98, No. 11, pp. 6500-6505. (2001). DOI: https://doi.org/10.1073/pnas.101545198

Liu S, Guo R, Simpson LG, Xiao Z-S, Burnham CE, Quarles LD. "Regulation of fibroblastic growth factor 23 expression but not degradation by PHEX". J Biol Chem. Vol. 278, No. 39, pp. 37419-26. (2003). DOI: https://doi.org/10.1074/jbc.M304544200

de Bono JS, Logothetis CJ, Molina A, Fizazi K, North S, Chu L, et al."Abiraterone and increased survival in metastatic prostate cancer". The New England journal of medicine. Vol. 364, No. 21, pp. 1995-2005. (2011). DOI: https://doi.org/10.1056/NEJMoa1014618

Formisano L, Stauffer KM, Young CD, Bhola NE, Guerrero-Zotano AL, Jansen VM, et al. "Association of FGFR1 with ERα Maintains Ligand-Independent ER Transcription and Mediates Resistance to Estrogen Deprivation in ER(+) Breast Cancer". Clinical cancer research : an official journal of the American Association for Cancer Research. Vol. 23, No. 20, pp. 6138-6150. (2017). DOI: https://doi.org/10.1158/1078-0432.CCR-17-1232

Chen YY, Li CF, Yeh CH, Chang MS, Hsing CH. "Interleukin-19 in breast cancer". Clinical & developmental immunology. Vol. 2013, pp. 294-320. (2013). DOI: https://doi.org/10.1155/2013/294320

Hsing CH, Cheng HC, Hsu YH, Chan CH, Yeh CH, Li CF, et al. "Upregulated IL-19 in breast cancer promotes tumor progression and affects clinical outcome". Clinical cancer research : an official journal of the American Association for Cancer Research. Vol: 18, No. 3, pp. 713-725. (2012). DOI: https://doi.org/10.1158/1078-0432.CCR-11-1532

Gangemi S, Minciullo P, Adamo B, Franchina T, Ricciardi GR, Ferraro M, et al. "Clinical significance of circulating interleukin-23 as a prognostic factor in breast cancer patients". Journal of cellular biochemistry. Vol. 113, No. 6, pp. 2122-2125. (2012). DOI: https://doi.org/10.1002/jcb.24083

Ishihara-Yusa S, Fujimura T, Lyu C, Sugawara M, Sakamoto K, Aiba S. "Breast Cancer Metastasis in the Skin with Hyperkeratotic Pigmentation Caused by Melanocyte Colonization". Case reports in oncology. Vol. 11, No. 3, pp. 660-664. (2018). DOI: https://doi.org/10.1159/000493186

Song N, Zhong J, Hu Q, Gu T, Yang B, Zhang J, et al. "FGF18 Enhances Migration and the Epithelial-Mesenchymal Transition in Breast Cancer by Regulating Akt/GSK3beta/Beta-Catenin Signaling". Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. Vol. 49, No. 3, pp. 1019-1032. (2018). DOI: https://doi.org/10.1159/000493286

Yu Z, Lou L, Zhao Y. "Fibroblast growth factor 18 promotes the growth, migration and invasion of MDAMB231 cells". Oncology reports. Vol. 40, No. 2, pp. 704-714. (2018). DOI: https://doi.org/10.3892/or.2018.6482

Ben-Aharon I, Levi M, Margel D, Yerushalmi R, Rizel S, Perry S, et al. "Premature ovarian aging in BRCA carriers: a prototype of systemic precocious aging?" Oncotarget. Vol. 9, No. 22, pp. 15931-15941. (2018). DOI: https://doi.org/10.18632/oncotarget.24638

Brooks AN, Kilgour E, Smith PD. "Molecular Pathways: Fibroblast Growth Factor Signaling: A New Therapeutic Opportunity in Cancer". Clinical Cancer Research. Vol. 18, No. 7, pp. 1855-1862. (2012). DOI: https://doi.org/10.1158/1078-0432.CCR-11-0699

Published

2020-12-20

How to Cite

The Estimate of Cytokines and Fibroblast Growth Factors in Patients with Breast Cancer. (2020). Journal of Zankoy Sulaimani - Part A, 22(2), 9-16. https://doi.org/10.17656/jzs.10803

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