Effect of Ficus carica leaves Aqueous Crud Extract on the Number of Wild Fruit Flies and Sperm Morphology of Laboratory Albino Male Mice


  • Mahmood Othman Ahmed Department of Biology, College of Science, University of Sulaimani, Kurdistan Region, Iraq. Author




Fig leaves extract, Drosophila melanogaster, Sex ratio, sperm abnormalities


The purpose of this study was to compare the effects of two concentrations of aqueous crude extract from Ficus carica leaves on fruit fly numbers, sex ratios, and generations, as well as laboratory albino mouse sperm abnormalities. Fruit flies were given the concentrations of 1% marked as C1 and 2% marked as C2 in culture media and allowed to complete their life cycle at an acceptable temperature (25o C±2) in an incubator. Same concentrations were given to the tested mice in drinking water. Each treatment consisted of five replications, with untreated mice and flies serving as the control groups. Fruit fly findings were seen in the first and second generations, and mouse sperm morphology was examined one month following treatment. The results revealed that tested concentrations had no significant impact (P≤0.05) on the quantity of fruit flies mean values were (40.650 ± 2.40 5and 39.250 ± 2.624) froe first and second concentrations compared to 34.550 ± 2.12 in control group, but they did have a significant impact (P≤0.05) on the sex ratio (shifted toward females (40.733 ± 1.969) in comparison to males (35.566 ± 1.838), least significant differences(L.S.D.) were = 5.148,  and generation-concentration interactions, the highest number were recorded was females in second generation (42.900 ± 2.203). The treated mice showed Significant effect of concentrations on sperm without tail and highly significant effect (P≤0.01) on sperm without hook (mean values were 5.6 ± 0.37 and 2.0 ± 0.44 in high concentration while in control group the values were 2.0 ± 0.44 and1.0 ± 0.44) for both aberrations respectively. Our results clearly indicated change in sex ratio among treated fruit flies and induction of some morphologic changes in mice sperms after treatment of fig leaves aqueous crud extract.


Ramaa CS, Shirode AR, Mundada AS, Kadam VJ (2006). Nutraceuticals: An emerging era in the

treatment and prevention of cardiovascular disease. Curr. Pharm. Biotechnol. Vol.7 No.(1):P.15-23.

Oyeyipo P., Y. Raji, and A. F. Bolarinwa, “Antioxidant profile changes in reproductive tissues of rats

treated with nicotine,” Journal of Human Reproductive Sciences, vol. 7, no. 1, pp. 41–46, 2014. DOI: https://doi.org/10.4103/0974-1208.130823

Shimaa A. Haredy, 2 Tamer S. Imam and 1Omar A. Ahmed-Farid (2017). Combination of Ficus Carica

Leaves Extract and Ubiquinone in a Chronic Model of Lithium Induce Reproductive Toxicity in Rats:

Hindrance of Oxidative Stress and Apoptotic Marker of Sperm Cell Degradation. OSR Journal of Pharmacy

and Biological Sciences. Volume 12, Issue 2:P 64-73.

Maha A. Fahmy, Entesar E. Hassan, Noha E. Ibrahim, Emad M. Hassan, Zeinab M. Hassan, Enayat A.

Omara (2020). Protective Role of Ficus carica Extract Against Hepato-Testicular Side Effects and

Genotoxicity Induced by Cisplatin. Pharmacogn journal, vol.12(3): 645-656.

Vikas, V. P. Bhangale, S. C. and Patil, V. R. (2010). Evaluation of anti-pyretic potential of Ficus

caricaleaves,” International Journal of Pharmaceutical Sciences Review and Research, .2 (2), 1-10.

Mawa S, Husain K, Jantan I. (2013). Ficus carica L. (Moraceae): Phytochemistry, Traditional Uses and DOI: https://doi.org/10.1155/2013/974256

Biological Activities. Evid Based Complement Alternat Med. 2013:974256.

Gibernau M, Buser HR, Frey JE, Hossaert-McKey M. (1997).Volatile compounds from extracts of figs DOI: https://doi.org/10.1016/S0031-9422(97)00292-6

of Ficus carica . Phytochemistry;46(2).P:241–244.

shurd O, Zgheel F, Kermagi A, et al. (2004). Microbial (l–3)-β-D-glucans from Libyan figs (Ficus carica DOI: https://doi.org/10.1016/j.carbpol.2004.06.040

) Carbohydrate Polymers;58(2).P:181–184.

Wongchum N, Dechakhamphu A. (2021). Ethanol extract of Cassia siamea L. increases life span DOI: https://doi.org/10.1016/j.bbrep.2021.100925

in Drosophila melanogaster. Biochem Biophys Rep.26;25:100925.

Hirofumi Nishizono, Masaki Shioda, Toru Takeo, Tetsumi Irie, Naomi Nakagata (2004). Decrease of

Fertilizing Ability of Mouse Spermatozoa after Freezing and Thawing Is Related to Cellular Injury, Biology

of Reproduction, Volume 71, Issue 3, 1.P:973–978.

Olga S. Arvaniti, Yiannis Samaras, Georgia Gatidou et al. (2019). Review on fresh and dried figs:

Chemical analysis and occurrence of phytochemical compounds, antioxidant capacity and health effects.

Food research international, Vol (119). P:244-267.

Cheryl S. Rosenfeld and R. Michael Roberts (2004). Maternal Diet and Other Factors Affecting

Offspring Sex Ratio. A journal article, Biology of reproduction, Vol.71 issue 4.P:1063-1070.

Pienaar J., Greeff J.M. (2003). Maternal control of offspring sex and male morphology in the Otitesella DOI: https://doi.org/10.1046/j.1420-9101.2003.00522.x

fig wasps.J Evol Biol Vol. 16. P:244–253.

Larson M, Kimura K, Kubisch H, Roberts R. (2001). Sexual dimorphism among bovine embryos in their

ability to make the transition to expanded blastocysts and in the expression of the signaling molecule IFNTau.Proc Natl Acad Sci U S A Vol (98)P:9677–9682. DOI: https://doi.org/10.1073/pnas.171305398

Bredbacka K, Bredbacka P. (1996). Sex-related cleavage rate difference in bovine embryos produced in DOI: https://doi.org/10.1016/0093-691X(96)84664-4

vitro is controlled by glucose.Theriogenology Vol. 45.P:191.(abstract)

Gutierrez-Adan A, Granados J, Pintado B, De La Fuente J. (2001). Influence of glucose on the sex ratio

of bovine IVM/IVF embryos cultured in vitro. Reprod Fertil Dev Vol.13.P:361–365.

Hamilton W. (1967). Extraordinary sex ratios. A sex-ratio theory for sex linkage and inbreeding has new DOI: https://doi.org/10.1126/science.156.3774.477

implications in cytogenetics and entomology.Science Vol.156 P:477–488.

Dyson EA, Hurst GDD (2004). Persistence of an extreme sex-ratio bias in a natural population. Proc Natl DOI: https://doi.org/10.1073/pnas.0304068101

Acad Sci U S A. ; Vol.101.P:6520–6523.

Umarqayum Abu Bakar, Puvaratnesh Subramaniam, Nurul Ain Kamar Bashah,Amira Kamalrudin ,

Malina Jasamai Wan Mohd Aizat, M. Shahinuzzaman and Mahanem Mat Noo (2020). Sperm Proteomics

Analysis of Diabetic Induced Male Rats as Influenced by Ficus carica Leaf Extract. Biotechnologyfor

sustainability and social well bein,Vol.8 issue (4).P:365-373.

Majid Naghdi, Maryam Maghbool, Morteza Seifalah-Zade,Maryam Mahaldashtian, Zohreh Makoolati,

Seyed Amin Kouhpayeh, Afsaneh Ghasemi, and Narges Fereydouni (2016). Effects of Common Fig (Ficus

carica) Leaf Extracts on Sperm Parameters and Testis of Mice Intoxicated with Formaldehyde. Hindawi

Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2016, Article ID

, 9 pages http://dx.doi.org/10.1155/2016/2539127. DOI: https://doi.org/10.1155/2016/2539127

Boukhalfa, Farid, et al. (2018) "Antioxidant activity and Hypolipidemic effect of Ficus carica leaf and

twig extracts in Triton WR-1339-induced hyperlipidemic mice." Mediterranean Journal of Nutrition and

Metabolism Vol.11(1),P: 37-50.

Odo, G. E., Agwu, J. E. , Newze, N. , Nwadinigwa, A. , Onyeke, C. C., Nzekwe, U. , Ajuziogu, G. C.,

Osayi, E. I. and Ikegbunam, C.(2016). Toxicity and effects of fig (Ficus carica) leaf aqueous extract on

haematology and some biochemical indices of wistar albino rats (Rattus norvegicus). Journal of Medicinal

Plants Research, Vol. 10(22), p: 298-305.

Nebedum J.O., Udeafor P.C. and Okeke C.U. (2010). Comparative effects of ethanolic extracts of Ficus

carica and Mucuna pruriens leaves on haematological parameters in albino rats. Biokemistri,vol.22 (2),P:77-

Ramezani M., Khalili M.A., Khoradmehr A.(2014). The study of Sperm quality after consumption of a

traditional herbal compound (adofon) by infertile men. Journal of animal biology, Vol 6 (3),P: 37 -44.

Lalu Ahmad Zaenuri, Trinil Susilawati, Sri Wahyuningsih, Sutiman Bambang Sumitro (2014). Effects of

Additional Crude Extract of Fig Fruit (Ficus carica L) into Tris Egg Yolk Based Extender on Quality of

Buck Semen. Journal of Biology, Agriculture and Healthcare, Vol.4(9),P:21-27.



How to Cite

Effect of Ficus carica leaves Aqueous Crud Extract on the Number of Wild Fruit Flies and Sperm Morphology of Laboratory Albino Male Mice. (2023). Journal of Zankoy Sulaimani - Part A, 25(2), 8. https://doi.org/10.17656/jzs.10929

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